Protective Function of the Recombinant Oshsp18.0-CII Protein, a Class II Small Heat Shock Protein of Rice, in Escherichia Coli

所有植物都會合成多種小分子量熱休克蛋白質。本研究在大腸桿菌（Escherichia coli）中大量表現Oshsp18.0-CII這個水稻18.0 kDa第二族小分子量熱休克蛋白質的基因，以研究其功能。結果顯示除了可因此提高大腸桿菌的耐熱性、且對大腸桿菌的水溶性蛋白質具有熱保護性之外，更可因此提高大腸桿菌對紫外光的耐受性，其對50°C一小時處理的耐熱性可比只轉入pET32a表現載體的對照組細胞增高幾乎1000倍的存活率；而對1700μJ的紫外光照射的耐性約為只轉入pET32a表現載體之對照組細胞的7.29倍。在900μJ的紫外光照射之後，未轉型的細胞幾乎無法存活，其存活率不高於0.4％。

關鍵字

熱休克；水稻；耐熱性；熱保護性；紫外光

並列摘要

All plants synthesize multiple families of small heat shock proteins (sHSP). A cDNA clone, Oshsp18.0-CII, encoding an 18.0 kDa class II sHSP was isolated from rice previously. The function of the rice class II sHSP was studied by overproduction of the Oshsp18.0-CII fusion protein in transformed Escherichia coli cells. The results suggest that heterologous expression of the Oshsp18.0-CII fusion protein increases thermotolerance of E. coli cells in vivo and provided thermoprotection to E. coli soluble proteins in vitro. The survival rate of Oshsp 18.0-CII fusion protein-accumulating cells treated at 50°C for 1 h was almost 1000-fold higher than that of the control cells transformed with pET32a expression vector. Overproduction of the Oshsp18.0-CII fusion protein in E. coli also confers tolerance of E. coli cells to ultraviolet (UV) irradiation. The post-UV survival of the Oshsp18.0-CII fusion protein-accumulating cells is about 7.29-fold over that of the control cells transformed with pET32a expression vector when exposed to 1700μJ of UV. There is almost no post-UV survival (≦0.4%) in the untransformed cells after exposing to 900μJ UV light.

並列關鍵字

Heat shock ； Oryza sativa L. ； Thermotolerance ； Thermoprotection ； Ultraviolet

國際替代計量

Protective Function of the Recombinant Oshsp18.0-CII Protein, a Class II Small Heat Shock Protein of Rice, in Escherichia Coli

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