Fusaric acid Induced Cell Death and Changes in Oxidative Metabolism of ＂Solanum lycopersicum＂ L

Vivek Kumar Singh ； Ram Sanmukh Upadhyay

《Botanical Studies》 55卷8月 (2014/08) Pp. h1-11

https://doi.org/10.1186/s40529-014-0066-2

並列摘要

Background: Fusaric acid (FA) has been shown to stimulate the rapid development of disease symptoms, such as necrosis and foliar desiccation. In this study, we have evaluated the phytotoxicity of FA on tomato plants (Solanum lycopersicum L.). FA induced necrotic lesions in detached leaves, which are reminiscent of hypersensitive response (HR) lesions induced by plant-pathogen interactions and other abiotic stress factors. Results: FA-treated tomato leaves exhibited visible necrotic lesion as a result of cell death which was evident by Evans blue staining, enhanced reactive oxygen species (ROS) levels and DNA degradation. Changes in the generation of O_2^- and H_2O_2 as well as the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were examined in FA-treated tomato leaves. It was observed that FA exposure stimulated oxidative burst in the leaves, resulting in a lasting activation of O_2^- and H_2O_2 production. After first day of FA application, the H_2O_2 scavenging enzymes CAT and APX showed a strong activity decrease followed by gradual recovery to the control level after 2 and 3 days. Conclusion: A concomitant increase in ROS production, the down regulation of antioxidative enzymes activities and upregulation of lipid peroxidation were crucial for the onset of cell death. These results suggested that FA-induced damage might result from ROS pathways. Thus, our experiments provide a useful model plant system for research on FA-induced plant cell death.

並列關鍵字

Cell death ； Fusaric acid ； Hypersensitive response ； Reactive oxygen species