Hypoxia-Inducible Factor-1α mRNA and Protein Levels in Renal Cell Carcinoma

Anders Lidgren ； Anders Bergh ； Kjell Grankvist ； Gudrun Lindh ； Börje Ljungberg

《Journal of Cancer Molecules》 4卷5期 (2008/12) Pp. 153-157

https://doi.org/10.29685/JCM.200812.0004

並列摘要

AIM: Angiogenesis plays a central role in the growth of a tumor. The hypoxiainducible factor 1α (HIF-1α) is a regulated subunit of HIF-1, a key factor that carries fundamental features in angiogenesis and tumor progression. Renal cell carcinoma (RCC) is highly vascularized with a variable clinical outcome, having specific genetic alterations in different RCC types. This study investigated HIF-1α mRNA and protein expression in relation to RCC type and clinicopathological variables including clinical stage and survival.
METHODS: Quantitative analysis of HIF-1α mRNA expression in 202 patients including 168 clear cell (cRCC), 23 papillary (pRCC) and 11 chromophobe RCCs. Additionally, 49 samples from non-malignant corresponding kidney cortex were analysed. Comparative analysis of HIF-1α protein expression was performed by immunohistochemistry of tissue microarray.
RESULTS: HIF-1α mRNA levels were significantly lower in cRCC compared with pRCC (P=0.001) and kidney cortex (P＜0.001). In cRCC, HIF-1α mRNA was correlated to gender and age. For pRCC there was no correlation between HIF-1α mRNA and tumor stage, nuclear grade, age, tumor size or gender. HIF-1α mRNA expression was inversely related to HIF-1α protein levels in pRCC (P=0.041) but not significantly in cRCC (P=0.075).
CONCLUSION: HIF-1α mRNA levels were significantly lower in cRCC copmpared with kidney cortex and the other RCC types. High HIF-1α protein expression appeared to suppress HIF-1α mRNA expression, distressing the HIF-1 pathway in RCC.

並列關鍵字

hypoxia ； HIF-1α ； RCC ； prognosis