Object: To assess the effectiveness of SanXianTuoLiDan on chronic fatigue syndrome and the effect of anti-fatigue in animals.Method: 60 patients with chronic fatigue syndrome received treatment with SanXianTuoLiDan. Different tests such as oxygen-tolerance, anti-fatigue and immune function has been performed as well as observation of the efficacy and safety.Result: The clinical observation shows SanXianTuoLiDan has significant efficacy on chronic fatigue syndrome. Clinical recovery rate is 46.67%, overall efficacy is 86.67%, and overall effectiveness is 100%. Pharmacological test shows the medication anti-fatigue, anti-anaerobic, enhancing and improving endurance and enhancing immunity.Conclusion: SanXianTuoLiDan has better anti-fatigue ability and is an effective medication for treating chronic fatigue syndrome.