- 期刊
萃取條件對香椿葉片總酚類物質萃取量的影響
Effect of Extraction on Phenolic Compounds Content of Chinese Mahogany Toona sinensis (A. Juss.) Roem. Leaves
摘要
本研究探究香椿（Toona sinensis （A. Juss.）Roem.）嫩葉酚類化合物分析條件。以50mL溶劑一次萃取時，0.25公克溶質萃取量最高，萃取量與溶質重之迴歸方程式為y=12.6299+(1.4605/x)，R^2=0.9429，p＜0.0001。在60℃下，以五種甲醇水溶液，即20%、40%、60%、80%、100%萃取，萃取所得總酚含量之與甲醇濃度間呈現極顯著之二次迴歸關係，迴歸方程式y=-0.0097(x-69.35)^2+38.7，R^2=0.9327, P＜0.001，顯示在69.35%的甲醇濃度下會有最大的萃取率38.7mg/g，60-80%間幾無差異。再進一步比較乾燥方法、萃取液成分與萃取條件對香椿葉片總酚類物質萃取量的影響。以真空冷凍乾燥、60%甲醇、萃取60分鐘可以獲得最高總酚類物質萃取量；但在60%甲醇萃取下萃取時間間差亦不顯著。因此，同時考慮對環境之衝擊，香椿嫩葉之酚類化合物萃取條件定為，0.25公克冷凍乾燥樣品，以50mL之60%甲醇水溶液，在60℃水浴下，萃取60分鐘。
並列摘要
This paper investigated total phenolic compounds extraction conditions of Toonasinensis (A. Juss.) Roem. leaf. Under once extraction by fifty mL solvent condition, extraction rate was high significant related to solvent/solute ratio, the regression formula was y=12.6299+(1.4605/x), R^2=0.9429, p＜0.0001, and 0.25 g solute had the best solute weight. Fine methanol content water solvent, that was 20%, 40%, 60%, 80% and 100% methanol, were used under 60 ℃ condition, extraction rate was high significant related by a quadratic regression model, the formula was y=-0.0097(x-69.35)^2+38.7, R^2=0.9327, P＜0.001, the theoretical highest extraction rate was under 69.35% methanol, but there had no significant difference between 60-80 % methanol. Comparison among three drying methods, the results shown that, freeze dry was the best, especially under 60 % methanol solvent in 60 ℃ for 30 minutes. The proper extraction condition should used 0.25 g freeze dry sample with 50 mL 60% methanol, and extracted for 30 minutes under 60℃ condition.