從斑馬魚與鯝魚分離抗菌胜肽cathelicidin相關之基因的片段

Isolation of Genomic Fragments of Cathelicidin Antimicrobial-Peptide Distal Related Gene from Danio rerio and Varicorhinus barbatulus

黃昱詠(Yu Yung Huang) ； 陳妍蓁(Yan Jen Chen) ； 謝萬璋(Wan Chang Hsieh) ； 張慧玫(Whei-Meih Chang)

《Journal of Information Technology and Applications(資訊科技與應用期刊)》 5卷4期 (2011/12) Pp. 176-179

https://doi.org/10.6302/JITA.201112_5(4).0004

摘要

The antimicrobial peptides (AMPs), generally consisting of 10~80 amino acids, are small molecules expressed by bacteria, plants and animals in their innate defense systems against invasive microbes. The cathelicidin gene belonging to the a-helical AMP family is well studied in humans and animals, however, less is known in fish. Hence, we choose to study the copy of this gene in Danio rerio and Varicorhinus barbatulus. The genomic DNA from these fish were isolated and used as templates targeted with cathelicidin-specific primer sets in DNA polymerase chain reaction. Four cathelicidin-related genomic fragments were obtained, two from zebra fish and two Varicorhinus barbatulus. The deduced open reading frames of these indicate low similarity ＜20% between their theoretical, antimicrobial regions. Higher similarity of 36% in amino acid sequence were aligned with cathelicidin genes from horse and porcine, suggesting common microbes were dealt with, common strategy were used, or limited data in short of species diversity found in APD database. To verify these, the completion of cathelicidin gene structural and functional analysis is currently under investigation.

關鍵字

cathelicidin基因 ； 斑馬魚 ； 鯝魚 ； APD資料庫

並列摘要

The antimicrobial peptides (AMPs), generally consisting of 10~80 amino acids, are small molecules expressed by bacteria, plants and animals in their innate defense systems against invasive microbes. The cathelicidin gene belonging to the a-helical AMP family is well studied in humans and animals, however, less is known in fish. Hence, we choose to study the copy of this gene in Danio rerio and Varicorhinus barbatulus. The genomic DNA from these fish were isolated and used as templates targeted with cathelicidin-specific primer sets in DNA polymerase chain reaction. Four cathelicidin-related genomic fragments were obtained, two from zebra fish and two Varicorhinus barbatulus. The deduced open reading frames of these indicate low similarity ＜20% between their theoretical, antimicrobial regions. Higher similarity of 36% in amino acid sequence were aligned with cathelicidin genes from horse and porcine, suggesting common microbes were dealt with, common strategy were used, or limited data in short of species diversity found in APD database. To verify these, the completion of cathelicidin gene structural and functional analysis is currently under investigation.

並列關鍵字

antimicrobial peptides ； cathelicidin ； Danio rerio ； Varicorhinus barbatulus ； APD database