- 期刊
A Novel Fermentation Related Protein from Mushroom, "Pleurotus Eryngiig", Might be Antibacterial
並列摘要
Increasing attention has being addressed to the urgent need of development of antimicrobial leads with novel structures because the worldwide emergence of antibiotic-resistant strains and the loss of efficacy of antibiotics to pathogens. A pET23a cDNA library from total RNA of 〞Pleurotus eryngiig〞 was constructed and screened for genes with antibacterial function by trypan blue as a vital stain. The main function of trypan blue dye is to test the loss of function of membrane permeation in dying cells. A positive clone Pe-5k was obtained. A substantially reduction of liquid cultural growth was driven by induced expression of clone Pe-5k. Sequence analysis of clone Pe-5k showed weak similarity in nucleotide BLAST but strong similarity in protein BLAST at NCBI database comparisons, suggesting an evolutionary importance of the overall structure and function.
延伸閱讀
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