The purpose of this study was to explore the effects of enhancing health physical fitness of mentally retarded students with Kinect for Xbox 360. The participants were two mentally retarded students. The single-subject experiment was adopted for which the A-B-A reversal design was used. Kinect was used as a sport intervention in this study. The eight-week sports training was processed 40 minutes each time, twice a week. Then, the researcher observed the change of students' health physical fitness in baseline period, intervention period, and fell back period. The results revealed that it could effectively improve mentally retarded students' health physical fitness with the intervention of Kinect for Xbox 360. Finally, according to the findings, the related suggestions for future studies were presented.