小海帶(Endarachne binghamiae)生態式室內量產苗源方式的研究

Seedling Mass Produced under Ecologically Artificial Cultivation in Endarachne binghamiae

郭漢鍠(Han-Hwang Gwo)

《國立虎尾科技大學學報》 27卷1期 (2008/03) Pp. 41-51

https://doi.org/10.6425/JNHUST.200803.0041

摘要

使用0.012%福馬林處理小海帶(E. binghamiae)同形配子後，培養密度維持在4.5±1.2×10^6個／1毫升過濾海水(1ccFSW)，然後分別置於光照度50μE•m^(-2) s^(-1)、不同溫度16±1℃、20±1℃及24±1℃、不同光週期10/14h(L/D)及14/10h(L/D)的條件下，發現16±1℃、20±1℃不管長短日照都比24±1℃長短日照附著率高，繼續培養又發現較高溫度24±1℃組已無法繼續發育。如將這些同形配子發育至絲狀體或盤狀體階段的小海帶再以打碎的方式又成為游離的單細胞，然後分別置於上述同樣培養的環境，發現同一光週期不論何種溫度平均附著率皆無差異，不過以同一溫度下比較，短日照與長日照即顯出差異。此附著後同培養條件繼續培養，發現再生發育至絲狀體與盤狀體的平均比例，在同光週期下，3種不同溫度下並無顯著差異，但同一溫度不同光週期，只有24±1℃下顯出差異，其餘兩組間無顯著差異；由實驗設計的培養環境下，發現較低溫度(16±1℃、20±1℃)下同形配子單爲生殖之附著及成長率較高，如絲狀體或盤狀體階段打碎後單細胞在短日照低溫(16±1℃、20±1℃)下再生成長也較好，這些的結果可提供小海帶(E. binghamiae)室內量產時培育苗源較佳環境因子的依據，也能克服該種季節環境上的限制。

關鍵字

小海帶 ； 同形配子 ； 單爲生殖

並列摘要

When Endarachne binghamiae isogametes are treated with 0.012% formaldehyde, maintaining at 4.5±1.2×10^6/1ccFSW, respectively placed in 50μE•m^(-2) s^(-1) of different temperature at 16±1, 20±1, 24±1℃, and of different photoperiod at 10/14h(L/D) and 14/10h(L/D), the adhesion rate of the 16±1℃and 20±1℃ groups are higher than that of 24±1℃group no matter how long the photoperiod is. In addition, the 24±1℃ group barely grow when continuously cultivated. Moreover, when the filaments or crustoses which parthenogenetically developed from isogametes are ground into single cells and placed under the above cultivation conditions, no obvious differences are found in the average adhesion rate in groups cultivated in the same photoperiod. On the other hand, cultivated under one temperature, big differences are found between the group of shorter photoperiod and the group of longer photoperiod. If continuously cultivated under the same condition, the average rates of regenerating into filaments and crustoses are not affected by the change of temperatures as long as it is cultivated under the same photoperiod. In comparison, when the cultivation temperature remains the same and the photoperiod differs, obvious differences are only found in 24±1℃ group. In short, the cultivation results reveal that the isogametes present higher adhesion rate and better parthenogenetic growth while crushed cells of filaments and crustoses regenerate better under shorter photoperiod at lower temperature(16±1、20±1℃). Hopefully, such observations may offer references to setting up finer cultivation environments for mass production and for overcoming the limitation of seasonal changes.

並列關鍵字

Endarachne binghamiae ； isogametes ； parthenogenetic growth

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