Studies on Antivenom Activity of ＂Ceiba pentandra＂ Leaves' Aqueous Methanol Extract Against ＂Echis ocellatus'＂ Snake Venom

Aqueous methanol of ＂Ceiba pentandra＂ leaves extract was tested for antivenom activity against ＂Echis ocellatus＂ snake venom. Among parameters investigated include: LD_(50) of the ＂Echis ocellatus＂ snake venom, phospholipase A_2 activity, percentage hemolysis. ＂In vivo＂ analysis of total protein content, white blood cells, pack cell volume and haemoglobin contents were also investigated. The result reveals that ＂Echis ocellatus＂ has an LD_(50) of 0.280mg/kg±0.065. Haemolysis due to venom has drastically reduced by the extract from 66% to 27.4%. suggesting that the extract is effective in reducing haemolysis in mice. The ＂in vivo＂ studies reveals that there were significant (p＜0.05) decrease in packed cell volume, total protein and haemoglobin contents for the venom group and there was only slight changes in the venom/extract and control groups suggesting that the extract has some inhibitory effect on the venom activity. The purified phospholipase incubated with the extract demonstrated neutralization effect against the phospholipase A_2 activity. The result has shown that ＂Ceiba pentandra＂ leaves extract possess potent snake venom-neutralizing capacity. The plant leaves extract could be use as an antidote for snakebite envenomation.

並列關鍵字

Antivenom ； Ceiba pentandra ； Echis ocellatus ； phospholipase A_2 ； plant ； snake venom

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