Effect of Aqueous and Ethanolic Extracts of ＂Ocimum gratissimum＂ on the Histology of the Spleen, Haematological Indicies of Wistar Rats and its Antimicrobial Properties

Abdullahi Shehu ； U.E. Umana ； J.A. Timbuak ； Q.W. Hamman ； S.A. Musa ； D.T. Ikyembe

《Asian Journal of Medical Sciences》 4卷4期 (2012/08) Pp. 130-133

並列摘要

The effects of the aqueous and ethanolic extracts of ＂Ocimum gratissimum＂ (Linn) on the haematological indices and histology of the spleen of adult Wistar rats, as well as its antimicrobial properties were investigated in this study. ＂Ocimum gratissimum＂ (Linn) is widely distributed in tropical and warm temperate regions and is commonly called ＂fever plant＂. Twenty five adult wistar rats with weight range of 180-220g were used. They were randomised into 5 groups. Group 1 was given normal saline 2mL/kg Group 2 and 3; 500mg/kg of aqueous and ethanolic extracts respectively while groups 4 and 5 1000mg/kg of aqueous and ethanolic extracts respectively. Administration was orally for 14 days. Pure strains of Escherichia coli, Staphylococcus aureus and Salmonella typhi were used for the antibacterial test with concentrations at 2 and 4% for each extract. The plates were then incubated at 37ºC for 24 h. The study showed that the extracts of ＂Ocimum gratissimum＂ had no antimicrobial effect at these concentrations; however, there was increased white pulp in the spleen more at concentrations of 1000 mg/kg for both extracts. Also, at 1000 mg/kg there was a significant increase in the RBC and lymphocyte count and a decrease in neutrophil count at (p＜0.05).

並列關鍵字

Haematological indices ； histology ； spleen ； wistar rats ； antimicrobial studies ； aqueous and ethanolic extracts and ocimum gratissimum