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- OpenAccess
Isolation and Screening of Keratinolytic Actinobacteria form Keratin Waste Dumped Soil in Tiruchirappalli and Nammakkal, Tamil Nadu, India
並列摘要
The aim of this study was to isolate keratinolytic Actinobacteria from feather dumping soil. Feather dumping soil was collected from several areas in Tiruchirappalli, Nammakkal, Tamil Nadu, and India. Twenty two isolates were selected after growth on Bennett's agar and they named as SD1 to SD22. All the twenty two isolates were subjected for primary screening on milk agar plates and among twenty two isolates ten were showing proteolytic activity in terms of making clear zone surrounding their colony on the Milk agar medium. The ten positive isolates were again subjected for the secondary screening on Feather Broth and three isolates, SD5, SD6 and SD7 were showing degradation of feather during their growth. Though the degradation process was taking long time, all these three isolates achieved complete degradation of feather between 20 to 25 days and they used feather as the sole organic source for carbon, sulfur and energy. These novel keratinolytic Actinobacterial isolates have potential biotechnological use in processes involving keratin hydrolysis.
延伸閱讀
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