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- OpenAccess
Effect of Nitrogen and Calcium Sources on Growth and Production of PHA of ＂Pseudomonas＂ sp. LDC-5 and its Mutant
並列摘要
Pseudomonas sp. LDC-5 is an indigenous isolate and its mutant MNNG-S produces Short Chain Length (SCL)-Medium Chain Length (MCL) PHAs when grown in unbalanced nutrient condition. Nutrient optimization studies would definitely pave way for large-scale production and there by facilitate commercialization. In this study both the strains were subjected to different nitrogen sources to identify the optimal nitrogen source and the influenze of Calcium chloride supplementation was examined. Mutant strain was found to produce higher and quicker yield compared to wild type amongst the minimal medium studied. Biomass and PHA yield was maximal through the use of Ammonium sulphate as nitrogen source. Calcium chloride supplementation was found to have a positive influence on both strains, in terms of growth rate and production. Time profile studies indicated that cellular biomass and PHA production are unified.
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