Molecular Identification of Avian Respiratory Viral Pathogens in Commercial Broiler Chicken Flocks with Respiratory Disease in Shiraz-Iran during 2009-2010

M. J. Mehrabanpour ； A. Rahimian ； A. H. Shoshtari ； P. D. Fazel ； E. Kariminejhad ； Gh R. Moazeni Jula

《International Journal of Animal and Veterinary Advances》 3卷5期 (2011/10) Pp. 300-304

並列摘要

Newcastle disease and avian influenza A viruses (AIV) have been responsible for serious losses in the Iran poultry industry. During 2009-2010 the poultry industry in Fars province faced an almost heavy loss that was characterized by mild and in some flocks high mortality and respiratory distress. The aim of this study was designed to clarify the roles of the Newcastle Disease Virus (NDV) and AIV singly and jointly by RT-PCR assay in recent outbreak in Fars province in Iran. During the period of this study a total 44 commercial broiler flocks with high mortality in Fras province were visited. Samples were collected from chickens with respiratory distress. To identify AIV and NDV by RT-PCR we used specific primers based on conserved sequences of the NP gene, HA gene and F gene. The results show, that out of 18 samples were positive for NDV, 6 for AIV and 18 flocks were infected with both NDV and AIV and 2 flocks of these were negative for AIV and NDV. It is most likely that flocks used in this study were also naturally exposed to a virulent strain of NDV which is why the vaccines were not covered. The high rates of AIV in broiler flocks in this study, confirms the endemic nature of AIV (H9N2) in Fars province.

並列關鍵字

Avian influenza ； avian respiratory disease ； broiler flocks ； Iran ； Newcastle disease ； RT-PCR