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- OpenAccess
Serological and Molecular Assays for Detection of Avian Influenza Virus in Broiler Chickens Flocks of Fars Province-Iran
並列摘要
H9N2 Avian Influenza Virus (AIV) is endemic in many Eurasian countries. Since 1998, this virus is pathogen of poultry industry in Iran. The aim of this study was to serological and molecular assays of H9N2 AIVs exposure of broiler chickens by the heamagglutination inhibition test (HI), Enzyme Linked Immunosorbent Assay (ELISA) test and RT-PCR technique in Fras province between November 2010 and March 2011. In the present study, tracheal tissues, cloacal swabs and serum samples collected from 30 broiler chicken flocks with respiratory disease symptoms in Fars province-Iran. The result of RT-PCR test showed that out of 24 flocks, for H9N2 AIVs were positive. The overall 300 serum samples were examined by HI and ELISA tests. In these tests, H7N7 and H5N1 AIV antibody (Ab) titers were negative in all flocks. Also, seroprevalence of H9 AIV subtype was positive in 263 and 274 serum samples by HI and ELISA test, respectively. The difference of Ab titers related to avian's immune system, age and many other factors. The results revealed the important role of H9N2 AIV in respiratory disease outbreak in commercial broiler farms of Fars province. In addition, the infectious flocks with high degree of mortality had high Ab titers against H9N2 AIV and it is show that low protective effects of Ab.
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