The Development of an Electrochemical Immunosensory for 1-naphthylacetic Acid

S.Z. Liang ； J.R. Pan ； X.M. Yin ； K. Sui

《Advance Journal of Food Science and Technology》 9卷6期 (2015/08) Pp. 439-443

並列摘要

A new detection method for 1-naphthylacetic acid (NAA) based on the electrochemical immunosensor was introduced in the study, in which anti-NAA polyclonal antibody was used. An monolayer-molecular, selfreassemble membrane was made from 3-mercaptopropionic acid on the surface of gold electrodes, on the membrane the anti-NAA antibody was immoblize through ester-activated method with addition of 1-(3-Dimethylaminopropyl)-3- ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS). Based on the three-electrode system, an NAA immunosenory detection method was established with using the signal from cyclic voltammetry. The standard curve could be represented as △E=0.0407lgc+0.0701(R^2=0.9984) with the range from 0.05μg/mL to 2μg/mL and with the detection limit was 0.035μg/mL. The sensory method had very low cross-reactivity, lower than 3%, to NAA similarities such as phenylacetic acid and indolyl-3-acetic acid. In addition, the sensor could be used with high stability as long as 30 days (room temperature). It indicated that a specific, stable electrochemical immunosensory detection method for 1-Naphthaleneacetic acid was successfully developed.

並列關鍵字

1-naphthylacetic acid ； electrochemical immunosensor ； self-assembled monolayer membrance