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Optimization of Enzymatic Hydrolysis of Protein from Small Yellow Croaker (Psendosciaena polyactis) Using Response Surface Methodology
並列摘要
Enzymatic hydrolysis of protein from small yellow croaker (Pseudosciaena polyactis) was studied by response surface methodology. Papain hydrolysate was found to have the greatest activity of antioxidant action of hydrolysate on DPPH. A central composite design with four independent variables: time (3, 4 and 5h); enzyme concention (0.2, 0.3 and 0.4%); temperature (50, 55 and 60°C); meal/water ratio (1:3, 1:4 and 1:5) was used to study the response variable (the degree of hydrolysis, DH). From RSM generate model, the optimum conditions for hydrolysis of protein from small yellow croaker were identified to be at temperature 57.75°C in 3.89 h reaction time, with enzyme concentration of 0.22% and meal/water ratio of 1:5. At the optimum conditions, predicted DH in the hydrolysis process was 49.37%.
延伸閱讀
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