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Development of an IMB-ELISA Method for Analyzing Pseudomonas fluorescens
並列摘要
To develop an immunemagnetic-bead-based enzyme-linked immunesorbent assay (IMB-ELISA), antisera were prepared12 from New Zealand white rabbits immunized with inactivated Pseudomonas fluorescens (P. fluorescens) and conjugated with magnetic-bead to get immunomagnetic-beads. IMB-ELISA would be developed through IMB collecting P. fluorescens. The results showed that the limit of detection ELISA and IMB-ELISA are, respectively 10^(1.1) cfu/mL and 10^(1.0) cfu/mL. The IMB-ELISA is more sensitive and higher efficient than IC-ELISA by calculating the recovery rate and the coefficient of variation, because of IMB quickly enriching the target bacteria. The IMB-ELISA is a quick and accurate method for the detection of P. fluorescens.
延伸閱讀
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