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Optimization Conditions of Production Fibrinolytic Enzyme from Bacillus lichniformis B4 Local Isolate
並列摘要
The study was conducted with the aim to found local isolate belongs to Bacillus lichniformis to produce fibrinolytic enzyme with highest activity under optimal conditions. Forty-five local isolates belongs to the genus Bacillus were selected for production of fibrinolytic enzyme (E.C. 3.4.). The isolate Bacillus lichniformis B4 was selected due to its high productivity of fibrinolytic enzyme. The optimal conditions for fibrinolytic enzyme production were determined, using a solid Lentils medium (activity 25.25 U/mL) at pH 7.2 (65.381 U/mL), 105 cell/g wet weight (19.185 U/mL) 48 h as incubation time (15.766 U/mL) and shaking incubator (95.992 U/mL) were the optimal culture condition for the production of greatest amount enzyme with highest activity. The optimal carbon and nitrogen sources were mannitol and peptone or soya peptone with activity 44 and 50 unit/mL, respectively.
延伸閱讀
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