Ectopic pregnancies occurring in the interstitial portion of the fallopian tube after ipsilateral total salpingectomy are rare. However, a careful diagnosis of the location of gestation in the following pregnancy is critical so as not to overlook a recurrent ectopic pregnancy after salpingectomy. This case report presents an unusual case of a laparoscopically treated spontaneous interstitial pregnancy that occurred 3 months after ipsilateral laparoscopic total salpingectomy. Early diagnosis of ipsilateral interstitial pregnancies after total salpingectomy is difficult. The treatment of interstitial pregnancies is inherently more challenging and difficult and it is associated with a high mortality rate compared with other ectopic pregnancies. Close monitoring of ensuing pregnancies in these patients is important and early diagnosis should be emphasized to avoid possible complications because of a delayed diagnosis.