光度、溫度及鹽度對螺旋藻超氧歧化酵素產量及抗氧化之影響

The Effects of Light Intensity, Temperature and Salinity on the Production of Superoxide Dismutase and Antioxidation of Spirulina Platensis

聶方珮(Fang-Pei Nieh) ； 孫明輝(Ming-Hui Sun) ； 林英才(Ying-Tsai Lin) ； 林雪良(Hsueh-Liang Lin) ； 鄭明得(Ming-Der Jeng) ； 彭清勇(Ching-Yung Pong)

《台北海洋技術學院學報》 5卷1期 (2012/03) Pp. 44-60

https://doi.org/10.29770/JTCMT.201203.0003

摘要

螺旋藻（Spirulina platensis）被發現含有高量抗氧化物、維生素A和β-胡蘿蔔素及超氧歧化酵素（superoxide dismutase, SOD）等，可幫助人體降低自由基的傷害，因此成為保健機能食品市場的熱門標的。本研究擬探討不同光照度、溫度及鹽度等培養條件對螺旋藻清除自由基DPPH及超氧陰離子活性的影響。實驗結果顯示最適螺旋藻培養條件為光照強度介於2000～4000 Lux、溫度介於30～37℃及鹽度0.1M以下之環境，培養42天後的螺旋藻最高生質產量可達6.0gL；此外，藻體甲醇萃取物清除自由基DPPH及超氧陰離子的能力測試結果顯示自由基清除率最高分別可達51.3%及34.5%，具有開發為高經濟與高營養價值保健品之潛力。

關鍵字

螺旋藻 ； 抗氧化能力 ； 超氧歧化酵素（SOD） ； NBT光還原反應法

並列摘要

Spirulina platensi has been the candidate for commercial high-quality health food, due to it has high content of antioxidant, vitamin A, β-carotenoid and superoxide dismutase (SOD). This study was focus at the effects of varied culture conditions including light intensity, temperature, and salinities on the growth and the anti-oxidative activity of Spirulina platensi. The results indicated that the optimum culture conditions for the growth of Spirulina platensi were as follows, 2000-4000 Lux of light intensity, 30-37℃ and lower than 0.1M of salinity. After 42 days cultivation, the max bio-mass will be more than 6.0 g/L in dry based. Additionally, the scavenging activity of the methanol extract of Spirulina platensi for DPPH and superoxide free radicals will be 51.3% and 34.5%, respectively. From this study, the Spirulina platensi could be provided as an effective antioxidant and further developed as health food.

並列關鍵字

Spriulina platensis ； antioxidative activity ； superoxide dismutase ； NBT test

參考文獻

黃鈴娟(2000)。樟芝與姬松茸之抗氧化性質及其多醣組成分析(碩士論文)。國立中興大學食品科學系。

鄭靖緹(2004)。鴻喜菇之液態菌種培養及功能性評估。南台科技大學化學工程研究所。

Ahamd, S.(1995).Oxidative Stress and Antioxidant Defenses in Biology.New York:International Thomaon PublishingInc.

Ayehunie, S.,Belay, A.,Baba, T. W.,Ruprecht, R. M.(1998).Inhibition of HIV-1 replication by an aqueous extract of Spirulina platensis (Arthrospira platensis).Journal of Acquired Immune Deficiency Syndromes and Human Restrvirology.18,7-12.

Belay, A.,Kato, T.,Ota, Y.(1996).Spirulina (Arthrospira): potential application as an animal feed supplement.Journal of Applied Phycology.8,303-311.