Ankle sprain is one of the most common injures in sports. The purpose of this study was to investigate the effects of base of support and vision on standing balance in healthy subjects and the subjects with functional ankle instability. Six healthy subjects and six subjects with functional ankle instability were recruited. Centre of pressure length was measured with a balance plate during standing in four different bases of support, standing with feet shoulder's width apart, standing with feet together, tandem standing and single-limb standing, and in two visual conditions, eyes-open and eyes-closed. In anterior-posterior direction, base of support and vision may be significant in postural control in the subjects with functional ankle instability but not in normal group. Stance with feet shoulder's width apart, stance with feet together, and eyes-open, showed lesser centre of pressure length in static standing. In dynamic standing, change of bases of support would be significant in the stable and unstable ankles. In medial-lateral direction, effects of base of support and vision could be more concern in static standing but not in dynamic standing. Understanding these two important human factors in stable and unstable ankles would be beneficial in developing effective intervention strategies targeting specific populations.