- 期刊
Effects of Magnesium Sulfate on Dynamic Changes in Blood Glucose Levels and Glucose Transporter-3 Expression in the Striatum during Short-term Forced Swimming in Gerbils
並列摘要
In a previous study, we reported that when gerbils were subjected to a period of forced swimming, glucose levels and magnesium levels in the plasma became elevated. In addition, a pretreatment with magnesium sulfate increased the expression of glucose transporter-3 (GLUT-3) in the cortex. The goal of the present study was to evaluate dynamic changes in blood glucose, lactate and magnesium levels in gerbils that had been subjected to short-term forced swimming using an auto-blood-sampling system. A second goal was to investigation the effects of forced swimming and magnesium sulfate on GLUT-3 expression, glucose and magnesium levels in the striatum, which is involved in energy delivery and motor function. Data collection involved the use of a microdialysis analyzer, a flame atomic absorption spectrometer and western blotting assays to detect glucose, lactate, magnesium and GLUT-3 levels, respectively. Gerbils were subjected to a blood sampling assay and pretreated with saline (n=6) or magnesium sulfate (n=6, 90 mg•kg-1, ip) 30 min before the period of forced swimming. Whole blood glucose, lactate, and magnesium levels increased to 175% and 235%, 305% and 190%, and 102% and 128% of basal levels during swimming in both the control and magnesium sulfate-treated groups (p＜0.05). The glucose and magnesium levels during swimming were 60% and 26% greater in the magnesium sulfate-treated group (p＜0.05), respectively, whereas the lactate levels were attenuated at 115% (p＜0.05). Another group of gerbils was subjected to a brain sampling assay. The gerbils were separated into four groups (n=4, each group): control group (C group) pretreated only with saline; magnesium sulfate group (Mg group) pretreated only with magnesium sulfate; saline control and forced swimming group (C+S group) pretreated with saline 15 min prior to the forced swimming period then rested for 30 min; the magnesium sulfate plus the forced swimming group (Mg+S group) was pretreated with magnesium sulfate 15 min prior to the forced swimming period then rested for 30 min. The results indicated that GLUT-3 and magnesium levels were slightly, but not significantly higher in the Mg group, the C+S group and the Mg+S group. These data suggest that magnesium increased whole blood glucose and magnesium levels during forced swimming; however, the effects of magnesium and swimming on GLUT-3 protein expression varied in different regions of the brain.
延伸閱讀
- Huang, C. Y., Liou, Y. F., Chung, S. Y., Lin, W. Y., Jong, G. P., Kuo, C. H., Tsai, F. J., Cheng, Y. C., Cheng, F. C., & Lin, J. Y. (2010). Role of ERK Signaling in the Neuroprotective Efficacy of Magnesium Sulfate Treatment during Focal Cerebral Ischemia in the Gerbil Cortex. The Chinese Journal of Physiology, 53(5), 299-309. https://doi.org/10.4077/CJP.2010.AMK063
- 張瀞云（2004）。Effect of Monosodium Glutamate on the Taste Cells of Circumvallate Papillae in Rats and Gerbils : An Immunocytochemical Study〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2004.02381
- Lin, M. C. (2008). Study on the Dynamic Changes of Extracellular Trace Elements and Energy Metabolites in Brain Cortex of Gerbil during Cerebral Ischemia and Reperfusion [doctoral dissertation, Kaohsiung Medical University]. Airiti Library. https://doi.org/10.6832/KMU.2008.00106
- Derkach, K. V., Bondareva, V. M., Chistyakova, O. V., Berstein, L. M., & Shpakov, A. O. (2015). The Effect of Long-Term Intranasal Serotonin Treatment on Metabolic Parameters and Hormonal Signaling in Rats with High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes. International Journal of Endocrinology, 2015(), 383-399-043. https://doi.org/10.1155/2015/245459
- Ferreira, L., Lemos, E. T. D., Pinto, F., Parada, B., Mega, C., Vala, H., Pinto, R., Garrido, P., Sereno, J., Fernandes, R., Santos, P., Velada, I., Melo, A., Nunes, S., Teixeira, F., & Reis, F. (2010). Effects of Sitagliptin Treatment on Dysmetabolism, Inflammation, and Oxidative Stress in an Animal Model of Type 2 Diabetes (ZDF Rat). Mediators of Inflammation, 2010(), 793-803. https://doi.org/10.1155/2010/592760