- 期刊
- OpenAccess
Index Evaluation of Anti-Oxidant Capacity and Anti-Aging Biological Activity of 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Parkinson's Disease in Mice
摘要
Parkinson's disease (PD) is one of the most common neurodegenerative diseases affecting approximately 1.5% of the world's population over 65 years of age. Clinically, the main symptoms include bradykinesia, stiffness, and tremors. The cause of this lack of motor function is due to the degeneration or death of dopamine-producing nerve cells in the brain. There are many possible factors that cause PD. At present, no drugs can be completely cured, and only symptoms can be delayed. In this study, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used for intraperitoneal injection to induce PD in C57BL/6 mice, which were randomly divided into normal group, selegiline-treated group and negative control group. Using commercially available ELISA kits and HPLC (high performance liquid chromatography) to detect dopamine content in brain striatum, antioxidant activity indicators in brain tissue and blood (Catalase, G6PDH (glucose-6-phosphate dehydrogenase), SOD (Superoxide dismutase activity), anti-aging bioactivity indicators [8-OH-dG (8-hydroxy-2-deoxyguanosine)], Protein carbonyl content, MDA (Malondialdehyde) content) and substantia nigra tyrosine hydoxylase (TH) antigenic expression. The results showed that the dopamine performance of the striatum was significantly higher in the selegiline-treated group than in the normal group. The control group; the three anti-aging biological activity indexes in brain tissue and serum were significantly lower in the selegiline-treated group and the normal group than in the negative control group; the antioxidant capacity index in the brain was significantly higher in the selegiline-treated group and the normal group. In the negative control group, the TH antigen-positive neurons in the substantia nigra pars compacta were significantly higher in the selegiline-treated group than in the negative control group. It is known from the results that this study has successfully established a mouse model of PD and drug screening systems, and in the future, this animal model can be used to provide basic research on PD lesions or PD drug and therapeutic strategy development.
延伸閱讀
- Huang, C. F. (2007). Studies on mechanisms of neurotoxicological effects of mercurial compounds in mice and offsprings after long-term and low-dose exposure [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2007.00076
- Kameda, T., Ohkawa, R., Yano, K., Usami, Y., Miyazaki, A., Matsuda, K., Kawasaki, K., Sugano, M., Kubota, T., & Tozuka, M. (2015). Effects of Myeloperoxidase-Induced Oxidation on Antiatherogenic Functions of High-Density Lipoprotein. Journal of Lipids, 2015(), 43-50. https://doi.org/10.1155/2015/592594
- 杜亞倢（2020）。The influence of perillaldehyde on anti-inflammation, anti-oxidation and lipid metabolism-related protein expression in diet-induced non-alcoholic fatty liver disease mice〔碩士論文，中山醫學大學〕。華藝線上圖書館。https://doi.org/10.6834/csmu202000187
- Bai, X. P., Qiu, A. Y., Guan, J. J., & Shi, Z. P. (2007). Antioxidant and protective effect of an oleanolic acid-enriched extract of A. deliciosa root on carbon tetrachloride induced rat liver injury. Asia Pacific Journal of Clinical Nutrition, 16(S1), 169-173. https://doi.org/10.6133/apjcn.2007.16.s1.32
- 林作慶、宋祖瑩、傅正思（2016）。The Ergogenic Effects of Ergothioneine Against Oxidative Stress and Muscular Damage Induced by Acute Exercise in Aging Rats。嘉大體育健康休閒期刊，15(2)，110-122。https://doi.org/10.6169/NCYUJPEHR.15.2.10