水產品中壬基酚之檢驗方法探討及其殘留量調查

Studies on the Analysis of 4-Nonylphenol in Seafood

張美華(Mei-Hua Chang) ； 顏維良(Wei-Liang Yan) ； 高雅敏(Ya-Min Kao) ； 施養志(Daniel Yang-Chih Shih)

《食品藥物研究年報》 1期 (2010/11) Pp. 87-98

https://doi.org/10.6945/ARFDR.201011.0087

摘要

本研究以超高效液相層析串聯質譜法(UPLC/MS/MS)分析水產品中壬基酚殘留量，檢體添加壬基酚13C6同位素標幟內部標凖品，以乙腈萃取，經正己烷去油脂及矽酸鎂層析匣淨化後，檢液以標準品添加法經UPLC/MS/MS測定其壬基酚殘留量。檢體中分別添加25、50及100 ppb之壬基酚，平均回收率為93.6~102.2%，變異係數為8.6~11.4%；本方法之檢出限量為10 ppb。以所建立之方法分析31件市售水產品，有4件檢出，其殘留量為14.5~49.4 ppb。

關鍵字

壬基酚 ； 液相層析串聯質譜法 ； 水產品 ； 標準品添加法

並列摘要

In this study, the residual 4-nonylphenol in seafood samples was analyzed by ultra performance liquid chromatography with tandem mass spectrometer (UPLC/MS/MS). Approximately 5 g sample was added to 13C6 isotope labeled n-nonylphenol as an internal standard, and then 4-nonylphenol was extracted with acetonitrile, partitioned with n-hexane saturated with acetonitrile, cleaned up with Florisil cartridge and quantified by UPLC/MS/MS with standard addition method. The average recoveries of 4-nonylphenol at the spike levels of 25, 50 and 100 ppb ranged 93.6~102.2%. The coefficients of variance were 8.6~11.4%. The detection limit of 4-nonylphenol was 10 ppb. Thirty-one seafood samples were analyzed by this method. Four samples were detected with residues of 4-nonylphenol ranging between 14.5 and 49.4 ppb.

並列關鍵字

4-nonylphenol ； UPLC/MS/MS ； seafood ； standard addition method