利用高效液相層析法定量化粧品中多種防曬劑成分

市面上的防曬化粧品種類相當的多，近年來民眾防曬的觀念逐漸普及，如何選用適當的防曬產品避免曬傷與曬黑成為重要的課題。在調查市售防曬化粧品之品質時，為能提高檢驗效率同時準確分析化粧品中的多種防曬劑成分，本研究擬建立一快速且再現性高之液相層析法，用以同時分析常見的9種防曬劑成分2-Phenylbenzimidazole-5-sulfonic acid、Benzophenone-3、Diethylamino hydroxylbenzoyl hexyl benzoate、Octocrylene、2-Ethylhexylp-dimethyl amino benzoate、Octyl methoxycinnamate、Butyl methoxydibenzoylmethane、Octylsalicylate及Homosalate。防曬劑標準品與檢體先經甲醇溶解萃取後，再以高效液相層析儀進行分析。使用之層析管柱為LiChrospher® 100 C18 （4.0 mm × 250 mm），移動相為甲醇與0.1%醋酸溶液以9：1 （v/v）之比例混合，流速為0.8 mL/min，標準品溶液與檢液注入量為20μL，分析時間20 min，檢測波長為310及350 nm。經實驗分析後，9種防曬劑所得標準曲線之線性回歸相關係數R2介於0.99605-1.00000之間，而各防曬成分在同日內及異日間分析所得結果之相對標準偏差分別介於0.02-6.93及0.03-6.51%之間。接著進行已知濃度的標準品添加後，求得此9種防曬成分之回收率介於82.42-118.34%，而其添加回收率之相對標準偏差則介於0.07-4.23%之間。進一步分析各防曬成分之最低定量濃度及最低檢測濃度，由實驗結果得知此9種防曬劑之最低定量濃度及最低檢測濃度分別介於0.002-0.005及0.0007-0.0015%之間。綜合以上結果，此篇研究中所建立之快速且再現性高之液相層析法，應該能同時準確地分析化粧品中常見的9種防曬劑成分，並應用於例行性之防曬化粧品品質檢驗。

關鍵字

2-Phenylbenzimidazole-5-sulfonic acid ； Benzophenone-3 ； Diethylamino hydroxybenzoyl hexyl benzoate ； Octocrylene ； 2-Ethylhexyl-p-dimethyl amino benzoate ； Octyl methoxycinnamate ； Butyl methoxydibenzoylmethane ； Octyl salicylate ； Homosalate ；高效液相層析法

並列摘要

A simplified quick and reliable high performance liquid chromatography (HPLC) method for the quantitative analysis of common sunscreen agents (2-phenylbenzimidazole-5-sulfonic acid, benzophenone-3, diethylamino hydroxybenzoyl hexyl benzoate, octocrylene, 2-ethylhexyl-p-dimethyl amino benzoate, octyl methoxycinnamate, butyl methoxydibenzoylmethane, octyl salicylate and homosalate) in cosmetics was developed in this study. Nine sunscreen standards and samples were extracted with methanol solution, followed by HPLC analysis. The HPLC was performed on a LiChrospher® 100 C18 (4.0 mm × 250 mm) column using methanol and 0.1% acetic acid (9 : 1, v/v) as the mobile phase at a flow rate of 0.8 mL/min and injection volume was 20 μL. The run time was 20 min and the chromatography was monitored by absorbance at 310 and 350 nm. The linear coefficients of regression equation of nine sunscreen agents were 0.99605-1.00000. The relative standard deviations of 9 sunscreen agents ranged between 0.02-6.93% (intraday) and 0.03-6.51% (interday). Recovery analysis was performed by spiking standard compounds into blank samples. The recoveries of 9 sunscreen agents were 82.42-118.34% and the coefficients of variation were 0.07-4.23%. The quantity limits and detection limits of 9 sunscreen agents were 0.002-0.005% and 0.0007-0.0015%, respectively. As a whole, this method was well qualified for the quantitative analysis of 9 sunscreen agents in commercial cosmetics.

並列關鍵字

2-phenylbenzimidazole-5-sulfonic acid ； benzophenone-3 ； diethylamino hydroxybenzoyl hexyl benzoate ； octocrylene ； 2-ethylhexyl-p-dimethyl amino benzoate ； octyl methoxycinnamate ； butyl methoxydibenzoylmethane ； octyl salicylate ； homosalate ； HPLC

國際替代計量

利用高效液相層析法定量化粧品中多種防曬劑成分

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