利用高效液相層析法同時分析化粧品中Salicylic acid、Zinc pyrithione、Climbazole、Piroctone olamine及Ketoconazole等抗菌成分

Simultaneous Determination of Salicylic acid, Zinc pyrithione, Ketoconazole, Climbazole and Piroctone olamine in Cosmetics by HPLC

潘德存(Te-Tsun Pan) ； 黃守潔(Shou-Chieh Huang) ； 陳玉盆(Yu-Pen Chen) ； 闕麗卿(Lih-Ching Chiueh) ； 施養志(Daniel Yang-Chih Shih)

《食品藥物研究年報》 4期 (2013/11) Pp. 325-331

摘要

本研究係利用高效能液相層析儀建立一簡單、快速的方法能同時分析化粧品中Salicylic acid、Zinc pyrithione、Climbazole、Piroctone olamine及列屬藥品成分管理之Ketoconazole等五種具抗頭皮屑效能之抗菌成分。檢體經由乙腈溶解定容過濾後，以高效能液相層析儀進行分析，層析管柱使用Agilent ZORBAX Eclipse Plus C18（150mm × 4.6mm），並採用乙腈、甲醇與50 mM磷酸二氫鉀（pH 2.5）作為移動相進行梯度沖提，以光二極體陣列檢出器偵測，檢測波長分別為230、300及340 nm。五種成分標準曲線之線性範圍為5-100 μg/ mL，線性回歸之判定係數（r^2）介於0.9998-0.9999之間，同日內及異日間試驗之相對標準偏差均＜2.60%。添加不同濃度之標準品於空白檢體中，回收率介於90.4-112.4%，相對標準偏差均＜1.3%。最低定量濃度則皆為0.005%。應用本研究所建立之方法檢測市售化粧品10件，結果均符合行政院衛生署公告化粧品中抗菌成分之容許限量，且均未檢出藥品成分Ketoconazole。

關鍵字

抗菌 ； 抗頭皮屑 ； 高效能液相層析

並列摘要

A high performance liquid chromatographic method was developed for simultaneous determination of salicylic acid, zinc pyrithione, ketoconazole, climbazole and piroctone olamine in cosmetics, using a Lichrospher Agilent ZORBAX Eclipse Plus C18 (I.D 15 cm × 4.6 mm, 5 μm) column. In order to survey the quality of marketed cosmetics, 10 samples were randomly collected by local health personnel in Taiwan area during the year of 2011. A mixture of 50 mM KH_2PO_4, methanol and acetonitrile was used as mobile phase by gradient elution. Flow rate was 1.0 mL/min and the chromatography was monitored by the absorbance at 230, 300 and 340 nm. The correlation coefficients, r^2, of regression equations of the five calibration curves were 0.9998-0.9999. The relative standard deviations (RSD) of the five estrogen ingredients for intraday and interday analysis were less than 2.60%. The average recoveries of these three ingredients spiked in sample ranged from 90.4 to 112.4%. The RSD’s of average recoveries were less than 1.3%. The limits of quantitation in cosmetics were 0.005% for salicylic acid, zinc pyrithione, ketoconazole, climbazole and piroctone olamine.

並列關鍵字

zinc pyrithione ； antibacterial ； salicylic acid ； HPLC