以高效液相層析儀及高效陰離子交換儀同步分析食品中葡萄糖、果糖、蔗糖、麥芽糖、乳糖及半乳糖檢驗方法之探討

Simultaneous Determination of Glucose, Fructose, Sucrose, Maltose, Lactose and Galactose in Foods Using High Performance Liquid Chromatography and High Performance Anion-Exchange Chromatography

古璦寧(Ai-Ning Ku) ； 吳白玟(Pai-Wen Wu) ； 陳瑋芸(Wei-Yun Chen) ； 沈盈如(Ying-Ru Shen) ； 黃子芸(Tzu-Yun Huang) ； 蔡沁玹(Ching-Hsuan Tsai) ； 張郁懷(Yu-Huai Chang) ； 曾素香(Su-Hsiang Tseng) ； 高雅敏(Ya-Min Kao) ； 周秀冠(Hsiu-Kuan Chou) ； 陳惠芳(Hwei-Fang Cheng)

《食品藥物研究年報》 6期 (2015/12) Pp. 22-35

摘要

衛生福利部公告於104年7月1日起，明訂「糖」為強制標示項目，故本研究探討以高效液相層析儀搭配揮發性光散射檢出器(HPLC-ELSD)或高效陰離子交換層析儀搭配脈衝電化學檢出器(HPAEC-PED)同步分析食品中葡萄糖、果糖、蔗糖、麥芽糖、乳糖及半乳糖。均質檢體以50%乙醇溶液萃取，於9000 × g離心後，取上層液，經適當稀釋，分別以HPLC-ELSD及HPAEC-PED進行分析。HPLC-ELSD測定條件為選用高耐水性胺基之Unison UKAmino管柱，移動相以去離子水及乙腈梯度沖提，流速為0.7 mL/min，柱溫60℃；HPAEC-PED測定條件為選用強陰離子交換之Dionex CarboPac PA20管柱，移動相以去離子水及100 mM氫氧化鈉溶液梯度沖提，流速為0.5 mL/min，柱溫30℃。以零卡可樂、餅乾、巧克力和奶粉為測試基質，經前述二種層析系統分析6種糖，均可於35分鐘內完成良好分離。上述零卡可樂等4種基質進行重複性及添加回收試驗，以HPLC-ELSD或HPAEC-PED分析6種糖之結果：測試基質分別添加10、20及40 mg/g之6種糖標準品，經HPLC-ELSD分析，其平均回收率介於83.1-119.9%之間，變異係數為0.6-11.0%；測試基質分別添加2、10及20 mg/g之6種糖標準品，經HPAEC-PED分析，其平均回收率介於74.8-123.7%之間，變異係數為0.4-11.7%。綜上，均符合本署食品化學檢驗方法確效規範。以此二法應用於3件市售產品含糖量之檢測，結果均於其營養標示值誤差允許範圍內(≦ 120%)。

關鍵字

食品 ； 糖 ； 高效液相層析儀揮發性光散射檢出器 ； 高效陰離子交換層析儀脈衝電化學檢出器

並列摘要

According to the new nutrition labeling launched on July 1^(st) of 2015, sugar content shall be labeled on packaged foods. This study aims to develop analytical methods for the determination of sugars including glucose, fructose, sucrose, maltose, lactose and galactose by high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and the high performance anion-exchange chromatography with pulsed electrochemical detection (HPAEC-PED). Each sample was extracted with 50% ethanol and then centrifuged. The supernatant was diluted with water and filtered before analysis. The HPLC-ELSD method was performed using Unison UK-Amino column. The gradient mobile phase consisted of acetonitrile and water was programed at a flow rate of 0.7 mL/min at 60℃. The HPAEC-PED method was performed using Dionex CarboPac PA20 column. The gradient elution consisted of 100 mM sodium hydroxide solution and water was operated at a flow rate of 0.5 mL/min at 30℃. Samples of coke zero, potato twist, chocolate and milk powder were spiked with different levels of sugars including 10-40 mg/g and 2-20 mg/g for HPLC-ELSD and HPLC-PED approach, respectively. The obtained recoveries were satisfied which gave 83.1-119.9% and 74.8-123.7% for HPLC-ELSD and HPLC-PED method, respectively. These developed methods were applied into a survey of three commercial samples purchased from retail stores. The results showed all samples complied with the nutrition labelling regulation.

並列關鍵字

Food ； sugars ； HPLC-ELSD ； HPAEC-PED