食品中反丁烯二酸二甲酯殘留之檢驗方法探討

Determination of Dimethyl Fumarate in Food by Gas Chromatography-Mass Spectrometry

郭景豪(CHING-HAO KUO) ； 方銘志(MING-CHIH FANG) ； 許哲綸(CHE-LUN HSU) ； 蔡佳芬(CHIA-FEN TSAIA) ； 高雅敏(YA-MIN KAO) ； 周秀冠(HSIU-KUAN CHOU) ； 陳惠芳(HWEI-FANG CHENG)

《食品藥物研究年報》 7期 (2016/12) Pp. 15-20

摘要

本研究建立以頂空固相微萃取技術(head space solid phase microextraction,HS-SPME)搭配氣相層析質譜法(gas chromatography / mass spectrometry, GC/MS)檢測食品中反丁烯二酸二甲酯(dimethyl fumarate, DMF)之殘留檢驗方法。檢體以頂空固相微萃取後進樣，改進了以往須以大量有機溶劑萃取之缺點，此外由於使用頂空分析，樣品不須經繁瑣的前處理步驟，節省了大量操作時間與溶劑。於食品檢體中添加適當標準溶液進行回收率及重複性等試驗。試驗結果，反丁烯二酸二甲酯之回收率為88.3 - 117.5% (添加濃度0.2及1 ppm)，變異係數均小於7%，定量極限為0.2 ppm。利用本方法進行市售10件糕點及10件麵包檢體之反丁烯二酸二甲酯殘留分析，檢驗結果，上述食品檢體均未檢出反丁烯二酸二甲酯。

關鍵字

氣相層析質譜儀 ； 反丁烯二酸二甲酯 ； 頂空固相微萃取

並列摘要

An analytical method for the determination of dimethyl fumarate (DMF) in food by headspace solid phase microextraction (HS-SPME) with gas chromatography/ mass spectrometry (GC/MS) was developed. HS-SPME is fast, simple and solvent-free, compared to conventional methods. A recovery study was performed by spiking standards into blank samples. The results showed great linearity of calibration. The recoveries of DMF were between 88.3 and 117.5% with coefficients of variation (CV) of less than 7% at 0.2 ppm and 1 ppm. The quantification limit of DMF was 0.2 ppm. A survey on the content of DMF in 10 cakes and 10 breads was done and all the samples were found negative for DMF.

並列關鍵字

GC/MS ； dimethyl fumarate ； headspace solid phase microextraction