Quantitative Analysis and Mass-Spectra Explanation for Diterpenoid Glycoside Atractyloside (ATR) in Traditional Chinese Herbs

陳良宇(Liang-Yu Chen) ； 陳智祥(Chi-Xiang Chen) ； 李慶國(Chin-Kuo Lee)

《MC-Transaction on Biotechnology》 3卷1期 (2011/03) Pp. 10-19

https://doi.org/10.29690/MCTB.201103.0002

摘要

現今以草本植物來治療疾病的情形十分普遍，這些草本植物有相當程度的藥用成分，但是同時含有毒性物質的情形也十分普遍，往往因食用不當導致中毒的情形屢見不鮮，例如服用含馬兜鈴酸之中藥而引起腎衰竭，因此了解這些植物中生理毒性風險的成份含量與發展便利可靠的檢測技術，是非常重要的課題。針對白朮（Atractylodes macrocephala）、蒼朮（Atractylodes lancea）等常用中藥，對於毒性成分－diterpenoid glycoside atractyloside（ATR）進行含量分析。樣品經水萃取後，酸化過夜後，以乙酸乙酯萃取出ATR成分，再以TMSI及pyridine衍生之後，以GC-MSD 進行分析。ATR衍生物之層析分離及質譜碎片被鑑定與解釋。定量結果顯示，蒼朮中ATR含量為8980 ppm，白朮中ATR含量為9230 ppm，另外也針對咖啡豆進行比對分析，發現微量ATR於咖啡豆。

並列摘要

Today, the ethnopharmacology is popular to treat disease, the herbs were used as considerable medicinal ingredients, but also the case with toxic substances is often due to improper poisoning, such as taking aristolochic acid caused kidney failure. Toxic risk evaluation of these plants in physiology is a very important issue to be facilitated by the reliable analytical method. Atractyloside (ATR) is one of a group of diterpenoid glycosides. The interest for these compounds was stimulated by the high toxicity of both glucosides, responsible of many deadly poisoning in past time. ATR occurs naturally in many Asteraceae plants, which are used as herbal medicines in Orient. The dried root of A. macrocephala and A. lancea are important crude drugs used in herbal medicine. ATR components were acidized and extracted from samples, then after the TMSI and pyridine derivatives for GC-MSD analysis. The ionic fragments in mass spectra were identified for TMS derivatives of ATR. The quantitative results showed that the ATR contents of 8980 ppm and 9230 ppm were detected in A. macrocephala and A. lancea, respectively. Others, the rare ATR was found in coffee beans for comparing.

並列關鍵字

Atractylodes macrocephala ； Atractylodes lancea ； atractyloside ； GC/MSD