Effects of Clinacanthus nutans (Burm.f) Lindau Leaf Extracts on Protection of Plasmid DNA from Riboflavin Photoreaction

核黃素光化學法檢測優遁草葉子萃出物對質體DNA的保護作用

苑舉民(Jeu-Ming P. Yuann) ； 王志玄(Jr-Shiuan Wang) ； 簡宏霖(Hong-Lin Jian) ； 林志璋(Chin-Chang Lin) ； 梁致遠(Ji-Yuan Liang)

《MC-Transaction on Biotechnology》 4卷1期 (2012/03) Pp. 45-58

https://doi.org/10.29690/MCTB.201203.0005

摘要

優遁草廣泛生長於亞洲熱帶地區，且是中國、馬來西亞、泰國重要的草藥。本實驗以綠茶作為對照，比較優遁草葉子萃出物的抗氧化能力及保護大腸桿菌的質體DNA的完整性。優遁草萃出物的抗氧化能力都較綠茶為低。綠茶的總多酚、SOD活性高於優遁草的30倍及10倍。但是，以核黃素光化學法檢測保護質體超螺旋DNA完整性的效果上，優遁草萃出物優於綠茶。

關鍵字

優遁草 ； 抗氧化 ； 超螺旋質體DNA ； 核黃素光化學法

並列摘要

Clinacanthus nutans (Burm.f) (CN) Lindau is widely grown in tropical Asia and is an important herbal medicine in China, Malaysia and Thailand. This study inspects the antioxidant activity and protective effects of Clinacanthus nutans extracts on plasmid DNA integrity in E. coli. The antioxidant activities of Clinacanthus nutans are lower compared with green tea. The superoxide dismutase (SOD) activity and total phenolic contents of green tea are almost 30- and 10-fold higher than that of Clinacanthus nutans leaves, respectively. However, Clinacanthus nutans leaf extract's retention of the integrity of super-coiled plasmid DNA under riboflavin photochemical treatment was shown to be better in comparison with extracts of green tea.

並列關鍵字

Clinacanthus nutans ； antioxidant ； super-coiled plasmid DNA ； riboflavin photochemical treatment