利用固定化本土性光合細菌顆粒在缺氧-好氧整合型生化反應器中同時去除水中氨氮及生產類胡蘿蔔素

Simultaneous Ammonium Removal and Carotenoids Production Using Immobilized an Indigenous Photosynthetic Bacteria Beads in an Anaerobic-Aerobic Hybrid Reactor

Jane-Yii Wu ； Mang-Chen Yu ； Duan-Wen Huang ； Wei-Hsun Lo

《國際創新發明大會》 2012 (2012/09) Pp. 173-179

https://doi.org/10.30079/CIISANNUAL.201209.0173

摘要

本研究自彰化地區之湖泊篩選多功能光合細菌株（Photosynthetic bacteria, PSB），此PSB菌株能在缺氧環境中生成類胡蘿蔔素，而在好氧環境下能直接去除氨氮。因此，不但可應用於水產養殖與畜牧業的廢水處理，又可同時生產高價值副產物，一舉兩得。本研究透過缺氧-好氧整合型生化反應器之研發，將反應器流動體積分為上升區與下降區，上升區注入氣體為好氧區，利用固定化菌體行固氮作用達氨氮去除之目的；下降區為缺氧區利用懸浮菌體達類胡蘿蔔素生產之目的。於缺氧-好氧整合型反應器條件參數操作下，顯示在下降缺氧懸浮菌體區，PSB菌量和類胡蘿蔔素含量分別可達到8g/L 和5 mg/g-dried cell weight；而在上升好氧固定化菌體區，PSB可達最高氨氮利用速率（25 mg/L/h）和100%氨氮去除效率（初始1000 mg/L）。另外，由PSB菌體所萃取出之類胡蘿蔔素，經過傅立葉轉換紅外線光譜和高效液相層析儀的分析比對後，證實其中一種為葉黃素（Lutein）。

關鍵字

光合細菌 ； 缺氧-好氧整合型生化反應器 ； 氨氮去除效率 ； 葉黃素和類胡蘿蔔素

並列摘要

In this study, an indigenous photosynthetic bacteria (PSB) was isolated from lake in Changhua. NH4^+ -N could be removed using immobilized PBS beads in the aerobic condition, and carotenoids (a high-valued product) production was carried out in the anaerobic. Therefore, simultaneous NH4+-N removal and carotenoids production using immobilized PSB beads in an Anaerobic-Aerobic Hybrid Reactor in this study. The experimental results showed that PSB biomass and carotenoids content reached 8 g/L and 5 mg/g-dried cell weight in anaerobic area, respectively. On the other hand, the NH4^+ -N utilization rate and NH4^+ -N removal efficiency of immobilized PBS beads were 25 mg/L/h and 100% in the aeration area, respectively. In additionally, one of the carotenoids is lutein in PSB, that was investigated by fourier transform infrared spectrometry (FTIR) and high-performance liquid chromatography (HPLC).