ICAM-1 and Fas Expression in Escherichia Coli O157:H7-associated Hemolytic Uremic Syndrome

大腸桿菌引發溶血性尿毒症候群中ICAM-1及Fas之表現

陳正穎(Chen-Yin Chen) ； 洪錦傳(Jin-Chang Huang) ； 蔡宗昌(Tsung-Chang Tsai) ； 李維仁(Wei-Jen Lee)

《東港安泰醫護雜誌》 11卷2期 (2005/06) Pp. 87-92

https://doi.org/10.7078/TSMHMNJ.200506.0087

摘要

目的：本篇文章分析溶血性尿毒症候群病人血中免疫系統指標，以了解溶血性尿毒症候群和免疫系統之關係。
材料與方法：分析十六位溶血性尿毒症候群之病人於發病急性期，中期及末期血液中T細胞和殺手細胞上CD54，CD95，CD69，CD25之表現。
結果：T細胞在急性期CD95，CD54之表現皆比對照組高並在病程末期回到正常。T細胞上CD69，CD25之表現則暫時升高後又下降。在殺手細胞上CD95，CD69之表現在急性期皆上升，之後隨著病程改善而下降。
討論：在溶血性尿毒症候群急性期，T細胞和殺手細胞有明顯之活化並伴隨細胞凋亡分子指標表現增加。

關鍵字

溶血性尿毒症候群 ； 細胞凋亡

並列摘要

Hemolytic uremic syndrome (HUS) due to Escherichia coli O157:H7 is a common cause of acute renal failure in children. We analyzed the immunological markers in these children and wish to realize the association between HUS and the immune system. The blood samples were collected on the first day of admission (acute phase), on the day of discharge when the patients' condition greatly improved (subacute phase) and one week later after discharge (convalescent phase). We quantitated ICAM-1(CD54), Fas (CD95), as well as activation markers (CD69, CD25) expression on their peripheral blood T cells and natural killer (NK) cells by flow cytometry. Ten age-matched healthy boys were included for comparison. We found that Fas and ICAM-1 expression on T cells in HUS patients during acute phase were greatly elevated compared to controls, both levels fell to control levels during convalescent phase. CD69 and CD25 expression on T cells, however, transiently increased then decreased thereafter. The elevated CD95 and CD69 expression on NK cells in HUS patients during acute phase also declined and were associated with clinical improvement. Thus, we demonstrated profound T and NK cells activation with enhanced ICAM-1 and Fas expression in acute HUS.

並列關鍵字

Hemolytic uremic syndrome ； Apoptosis