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光照條件影響嗜熱藍綠菌在平板反應器固碳及藻藍素生產之研究

Cultivation of Thermosynechococcus sp. with flat plate photobioreactors for CO_2 fixation and phycocyanin production affected by various light conditons

摘要


本研究選定乙株耐熱藍綠菌Thermosynechococcus CL-1 (TCL-1) 在平板式光合反應器(flat plate photobioreactors , FPPBR)進行高密度培養,並進行光不同光源及光強度對生質體產量、二氧化碳固定及藻藍素(phycocyanin, PC)產量之影響。研究結果顯示在此FPPBR 中皆有相當高的產量。在以白光培養至12 小時,可得最大的生質體產量及固碳量分別為0.82 gL^(-1) 12h^(-1) 及1.29 g L^(-1)。在紅光的培養下無助於這二項目標的提昇,與白光條件皆呈現約2%的PC 含量。而當光暗的(12L/12D)循環下的暗條件,TCL-1 約有0.1 至0.2 g L^(-1) 的生質體減少量及2.6%的PC 含量的下降。大的PC 含量5.3%發生於1,000 μE m^(-2)s^(-1) 白光LED 的照光培養條件下。因此,在固碳及PC 產量的考量下,在進入暗條件前進行收成為最佳的時機。

並列摘要


A thermophilic cyanobacterium named Thermosynechococcus CL-1 (TCL-1) was cultivated in flat plate photobioreactors (FPPBR) with dense culture to examine effects of light source and illumination intensity on biomass production, CO_2 fixation, and phycocyanin (PC) production. It showed that biomass production, CO_2 fixation, and PC production could be resulted in great productivity with FPPBR. The greatest biomass productivity and CO_2 fixation rate were 0.82 gL^(-1) 12h^(-1) and 1.29 g L^(-1) with white LED illumination within 12 hours, respectively. Even so, it showed no benefit to biomass production and CO_2 fixation with red light LED. The content of PC was about 2% in TCL^(-1) with white or red light LED illumination. When light dark cycle was applied, the biomass of TCL-1was reduced about 0.1 to 0.2 g L^(-1) and the content of PC was reduced about 2.6% during the same dark period of 12 hours. The maximum content of PC for TCL-1 is 5.3% with 1,000 μE m^(-2)s^(-1) white LED illumination. The appropriate harvest time determines the harvested content of PC of TCL-1 and the time before darkness was best time to harvest TCL-1 for the PC of TCL-1 is very valuable.

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