The effects of steam heating, addition of sugar and citric acid on banana puree (filling) charcaterics were studied. Regarding to the physicochemical analysis of the steam-heated banana puree, the residual activity of PPO was 2.70% and the residual activity of POD was 4.17% after banana heating for 8 min. The residual activity of PPO was 0.9% and the residual activity of POD was 0% after banana heating for 10 min. Both enzyme activity was inhibited, thus banana b value (yellowness) was higher after steam heating treatment. The banana puree showed more banana aroma and flavor after steam-heated for 8 min, and this processing condition was suitable for banana filling processing. Adding sugar increased the total soluble solids, and decreased water activity. Sugar addition from 0% to 40%, the water activity of banana filling decreased from 0.934 to 0.591, and it was apparently showed bright yellow and full of aroma. In our studies, banana cakes adding 40% sugar and 0.4% citric acid showed highest hedonic sensory overall acceptability.