光照及礦物營養對水稻幼苗核糖核酸分解酶活性之影響

Effect of Light and Mineral Nutrition on the Ribonuclease Activity of Rice Seedlings.

邱全芊(Chyuan-Chian Chiou) ； 童武夫(Wu-Fu Tong)

《師大生物學報》 25卷 (1990/11) Pp. 23-30

https://doi.org/10.29812/BBNTNU.199011.0003

摘要

水稻幼苗在生長發育過程中，其形態及核糖核酸分解酶活性，都會受光照的影響；光照幼苗的酶活性隨著幼苗的生長而急速增高，而缺乏光照的幼苗酶活性增幅較低。礦物營養的供應不論是對光照或黑暗生長幼苗之酶活性都有促進的作用，其中以對光照幼苗酶活性的促進最為顯著。電泳分析核糖核酸分解異構酶，至少可觀察到七條異構酶帶。B群異構酶活性完全不受光照或礦物營養之影響，故可能為水稻的基本異構酶。A1、A4、及C異構酶活性，則明顯地受光照及礦物營養之促進。由於A2、A3、異構酶祇見於黑暗生長之幼苗，顯示光照可完全抑制此兩異構酶之產生。

關鍵字

核糖核酸分解酶 ； 同功異構物 ； 礦物營養

並列摘要

Light irradiation affected obviously the morphogenesis and the ribbonuclease activity of growing rice seedlings. Increase of enzyme activity with aging of light-grown seedlings, while only a small amount activity increased of dark grown seedlings were observed. Enhencement of enzyme activity by the supplement of mineral nutrition were show in both light and dark grown seedlings, unexpectively the enzyme activity was raised to over two-fold in light-grown seedlings. At least seven isozymes could be clearly analyzed by native gel electrophoresis. Activity of B group isozymes were totally unaffected by the treatment of both light and mineral nutrition, thus these B group isozymes appears to be the constitutive enzyme of the seedling. Activity of A1, A4 and C isozymes were induced sensitively either by light or mineral nutrition. Since A2 and A3 isozymes showed only in the dark-grown seedlings, it indicates that the expression of these two isozymes was completely inhibited by light.

並列關鍵字

ribonuclease activity ； native gel electrophoresis ； isozyme ； light irradiation ； mineral nutrient