胞內傳訊途徑對B淋巴球功能的影響

Effect of Intracellular Signals on Immnoglobulin A Secretion in Murine B Lymphocytes

謝佳珍(Jia-Jen Shieh) ； 劉倩君(Chian-Jiun Liou) ； 曾哲明(Jer-Ming Tseng)

《師大生物學報》 27卷 (1992/06) Pp. 71-82

https://doi.org/10.29812/BBNTNU.199206.0007

摘要

A型免疫球蛋白是人體黏膜免疫系統B淋巴球分泌的主要抗體，但是導致黏膜系統之B淋巴球分泌IgA的機制尚無定論。本研究報告旨在討論是否cAMP，cGMP，Ca2+及PKC是否涉及IgA分泌之調節，實驗結果顯示，兩種誘導cAMP增加之藥物，prostagland in E2和forskolin抑制脾臟B淋巴球生長及IgA之分泌，而誘發cGMP增加之藥物(Lukotriene B4)對細胞生長及IgA分泌沒有影響，利用Ionophore A23187增加胞內鈣離子濃度減少了活細胞數目IgA之分泌，相反地，以活化PKC之藥物phorbolmyristate acetate(PMA)處理B淋巴球，發現.PMA促進細胞，增生且增加細胞活性，但PMA不影響IgA分泌，由於以上四種處理皆無法誘導IgA之分泌，所以真正影響IgA之分泌胞內傳訊途徑有待進一步之研究。

並列摘要

Immunoglobulin A (IgA) is the major immunoglobulin isotype secreted by mucosal lymphoid B cel1s. The mechanisms that induce mucosal lymphoid B cells to secret IgA are not known. This report examines whether the well-defined intracellular signals, including cyclic AMP, cyclic GMP, Ca2+ and protein kinase C (PKC), are involved in inducing IgA secretion. Experimental results show that prostaglandin E2 and forskolin, two potential cyclic AMP stimulators, suppresses growth and IgA secretion of murine splenic B cells. Leukotriene B4' a cyclic GMP inducer, has no effect on either growth or IgA secretion. An increase in intracelular Ca2+ concentration by treating the cells with ionophore A23187 reduces both cell number and IgA secretion. Activating PKC directly by phorbol myristate acetate (PMA), on the contrary, enhances cel1 pro1iferation and increases cell viability. However, PMA dose not affect the level of IgA secretion. Further studies are needed to examine other intracellular signal transduction pathways for the induction of IgA secretion.