中藥茯苓對老鼠B淋巴球功能的影響

Effect of a Chinese Herbal Medicine, Fu-Ling on the Production of Immunoglobulins from Murine Splenic B Lymphocytes

賴怡琪(Yi-Chi Lay) ； 劉倩君(Chian-Jiun Liou) ； 曾哲明(Jer-Ming Tseng)

《師大生物學報》 28卷 (1993/06) Pp. 53-63

https://doi.org/10.29812/BBNTNU.199306.0007

摘要

本文針對中藥材「茯苓」對老鼠B淋巴球分泌免疫球蛋白（抗體）功能及其生長之影響加以研究，發現不論是茯苓生藥或朱拌炮炙品之甲醇淬取物，對B淋巴球皆有胞殺作用，其存活率降低之程度隨茯苓萃取液增加而增加，在培養基中含50%茯苓萃取液之下，B淋巴球在24小時內由90%存活率降至20%，到培養第四天為止保持20%左右之存活率，至第五天才降至0%，IgG及IgM分泌隨總細胞數及存活率之降低而減少，但IgA總分泌量則不因茯苓萃取液在培養基中之含量增加而明顯減少，以單位細胞數（即10^6個B細胞）之分泌量而言，不論生藥或炮炙品萃取液皆顯著促進IgA分泌的增加。由本實驗結果推論得知，茯苓與其他增進免疫功能之藥材配合使用，旨在調和其他藥材之藥效，使免疫系統不至過於亢進，茯苓選擇性促進IgA之分泌則有利於增強黏膜免疫之功能。

關鍵字

茯苓 ； 老鼠B淋巴球 ； 免疫球蛋白

並列摘要

Fu-Ling, the sclederma of Poria cocos (Schw.) Wolf, has long been used as a sedative and diuretic in Chinese traditional medicine. In this report, the effect of Fu-Ling extract on both viability and immunoglobulin production of murine B lymphocytes were studied. Fu-Ling, raw material or processed product, was extracted with 50% methanol. The extract was dried and redissolved in B-cell culture medium. Murine splenic B lymphocytes were then cultured in the media containing various amounts of Fu-Ling extract. Fu-Ling extract dose-dependently decreased both total cell number and viability of B lymphocytes. Medium containing 50% of Fu-Ling ex-tract reduced B-cell viability from 90% to 20% in 24 hours, and then the B-cell viability showed little change in the following culture peroid. However, both cultures with and without drug showed spontaneous decrease in viability after day 5. The effects of Fu-Ling extract on IgG and IgM secretion corresponded well with the decrease in B-cell viability. Fu-Ling extract showed dose-dependent reduction of IgG and IgM secretion. However, IgA secretion was not significantly reduced by the presence of Fa-Ling extract in culture medium. Based on the amount of IgA secretion per 10^6 B-cells, we obseved a significantly increase in IgA secretion induced by Fu-Ling extract after three days of culture. The augmented effect of Fu-Ling on IgA secretion could be demonstrated by either extract from raw material or processed product. The data suggested that Fu-Ling might suppress the level of IgG and IgM antibodies by reducing the number of viable B lymphocytes but it could selectively increase the level of secretory IgA to enhance the function of mucosal immune system.

並列關鍵字

Fu-Ling ； murine B lymphocyte ； immunoglobulin