吉貝素、細胞分裂素及真菌抽出物對水稻細胞澱粉酶之誘導

Induction of Amylase by Gibberellin, Benzyladenine, and Fungal Elicitor in Suspension-Cultured Rice Cells

周瑞琪(Rui-Chi Chou) ； 童武夫(Wu-Fu Tong)

《師大生物學報》 28卷 (1993/06) Pp. 65-74

https://doi.org/10.29812/BBNTNU.199306.0008

摘要

水稻懸浮細胞對環境因子刺激之反應為本研究之探討重點。吉貝素（Gibberellin）和細胞分裂素（Benzylaldenine）可以促進細胞的生長，但不能延後細胞的老化。這兩種植物調節素都能明顯誘導α-及β-澱粉酶的合成及釋出，其中以吉貝素的誘導作用較佳。以葡萄糖為唯一碳源時，並不會抑制澱粉酶的被誘導。顯示植物調節素的刺激，會使水稻細胞的代謝作用增強，而反應於細胞的生長及澱粉酶的釋出。真菌抽出物（Fungal elicitor）的處理，也能誘導培養基中澱粉酶活性增高，同時也促進細胞內過氧化酶及苯丙胺酸去胺酶活性增強。顯示真菌抽出物誘發了細胞的防禦機制，促使一些有關的基因表現，而提高了細胞的代謝活性。

關鍵字

水稻細胞 ； 澱粉酶誘導

並列摘要

Responses of cultured rice cells ot the changes of environmental condition were investigated Cell growth was promoted by addition of either gibberellin or benzyladenine to 10 uM, but neither growth regulator could prevent cells from being senescent The biosynthesis and secretion of α- and β-amylase could also induced by treatment of the two phytohormones, and between them gibberellin appeared to be more effective than benzyladenine. Replacement of sucrose by glucose as the main carbon nutrient could not diminish the inductive effect of the phytohormones. These results indicate that gibberellin and benzyladenine are able to cause cell growth and biosynthesis of amylases. Treatment of fungal elicitor also stimulated rice cells to secrete more amylases and, in addition, caused an increase of the activities of peroxidase and phenylalanine ammonia-lyase in the cell. It is concluded that the expression of certain genes concerning to the defense mechanism might be induced by treatment of fungal elicitor, from which the metabolic activity of cells are elevated.

並列關鍵字

rice cells ； amylase induction