茯苓對人體血液淋巴球分泌免疫球蛋白的影響

Effect of Fu-Ling on Immunoglobulin Secreted by Lymphocytes in Human Blood

呂丹妮(Tan-Ni Lu) ； 余淑絹(Shwu-Jiuan Yu) ； 曾哲明(Jer-Ming Tseng)

《師大生物學報》 29卷1期 (1994/06) Pp. 43-51

https://doi.org/10.29812/BBNTNU.199406.0006

摘要

茯苓對人體血液淋巴球分泌免疫球蛋白的影響茯苓為中醫藥方劑中使用很廣的藥材，具有利水滲濕補氣健脾及安神之功效，本實驗則探討茯苓之50%熱乙醇萃取液，對人體週邊血液B淋巴球分泌功能之影響。實驗結果顯示，茯苓生藥及朱拌炮炙品的萃取液中之成份，顯著調節B淋巴球免疫球蛋白之分泌，其中IgM之分泌，在細胞處理藥物4天後，呈顯著之增加，而IgE之分泌在相同條件之下，則呈顯著之減少，此外，IgA之分泌在生藥萃取液處理下，呈顯著但輕微之增加（p=0.038）。IgG之分泌量不受茯苓萃取液之影響。實驗數據也顯示，茯苓或朱茯苓之萃取液並不影響人類單核球的生長與活性。以矽膠色層分析之結果，顯示促進IgM分泌之成份分佈在極性較低及中極性的成份群中，而抑制IgE分泌之成份則集中在低極性成份群中。顯然造成IgM分泌增加及抑制IgE分泌之因子並不相同，而IgM之促進因子可能不只一種。本研究結果證明茯苓確實對人類免疫系統之功能有調節作用。

關鍵字

茯苓 ； 人體B淋巴球 ； 免疫球蛋白

並列摘要

Fu-Ling, the sclederma of Poria cocos (Schw.) Wolf, has long been used as a sedative and diuretic in Chinese traditional medicine. However, the previous documents demonstrated that Fu-Ling extract showed regulatory effects on human immune cells. In this report, the regulatory effects of Fu-Ling extract on the secretory functions of human peripheral B lymphocytes was studied. The experimental results showed that both raw material Fu-Ling extract and mercury oxide-processed Fu-Ling extract sigificantly enhanced (p＜0.001) IgM secretion of human B-cells in a dose- dependent manner four days after the treatment. IgE secretion, by contrast, was signific antly suppressed (p＜0.005) by the Fu-Ling extract. IgA secretion was slightly but significm antly affected by the Fu-Ling extract (p＜0.05) and IgG secretion was unaffected. Throughout the study, we did not find significant effect of Fu-Ling extract on both cell growth and viability of monocytes and B lymphocytes. Results obtaining from the Silica gel chromatography showed that IgM was induced by more than one fraction which was eluted by the solvents with relatively low polarity and moderate polarity. However, IgE suppressor was eluted by a solvent relatively low polarity. This study demonstrated that Fu-Ling did have profound effect on human B-cell functions.

並列關鍵字

Fu-Ling ； Human B-lymphocyte ； Immunoglobulin