臺灣產褸網蜘蛛分子遺傳結構之分析

RAPD-Estimated Genetic Relationship of Psechrid Spiders (Araneae: Psechrus) in Taiwan

林俊宏(Chun-Hung Lin) ； 李錦年(Chin-Nien Lee) ； 楊智傑(Chih-Che Yang) ； 呂柏緯(Bor-Wei Lue) ； 陳世煌(Shyh-Hwang Chen) ； 呂光洋 ； 方剛(Kang Fang)

《師大生物學報》 34卷2期 (1999/12) Pp. 95-104

https://doi.org/10.29812/BBNTNU.199912.0001

摘要

遷移能力低的篩疣類褸網蜘蛛雖然在臺灣處處可見，但臺灣地形受到高山與短促河流分割，造成地形阻隔，可能因此產生族群遺傳結構的變化。本文使用隨機多型性放大（Random amplification of polymorphism determination; RAPD）方式，以不同的隨機引子放大DNA模板之後，分析分佈於臺灣北、中、南及東部的褸網蜘蛛，對各地旅群的基因結構作探討。結果顯示臺灣褸網蜘蛛分兩個群組，分別為北部及中部群組與東部及南部群組，初步結果也顯示褸網蜘蛛的族群遺傳結構與地理隔離相關。這種變化是否是因為地理隔離產生或是本島褸網蜘蛛來自不同的祖先，因地緣阻絕隔離而產生分化，仍然需要更多的相關研究以證實。

關鍵字

隨機多型性放大 ； 褸網蜘蛛

並列摘要

RAPD analyses were performed to eight different populations of Taiwanese psechrid spiders to determine their genetic relationships. An overall of sixteen primers giving rise to 174 polymorphic bands were used to examine nucleotide similarities and an UPGMA tree was constructed from the data obtained. The results showed that the population of psechrid spiders in Taiwan can be grouped into several clusters that have a high level of genetic heterogeneity. Samples collected from northern and central Taiwan can be paired with those from southern and eastern sectors of the island that indicates genetic variation among the populations studied because of geological separation. More in-depth investigation is needed before a more clear picture on the genetic structure of psechrid spiders in Taiwan be finely understood.

並列關鍵字

RAPD ； Psechrid spiders