食用紅甘蔗核糖體基因內轉錄間隔區之選殖與分析

The Cloning and Analysis of Internal Transcribed Spacer of Ribosomal DNA of Saccharum officinarum L.

陳裕星(Yu-Hsin Chen) ； 曾勝雄(Sheng-Hsiung Tseng) ； 沈勳(Sheen Schen)

《臺中區農業改良場研究彙報》 73期 (2001/12) Pp. 65-77

https://doi.org/10.6956/BTDAIS.200112.0065

摘要

本研究以引子5'-GTC CCT GCC CTT TGT ACA-3'；5'-CGC CGT TAC TAG GGG AAT CCT-3'可順利選殖出紅甘蔗部份核糖體基因及全長之內轉錄間隔區，長度共821鹼基，其中包括18S基因142鹼基，ITS1 206鹼基，5.8S基因164鹼基，ITS2 217鹼基以及26S基因92鹼基。依照ITS序列，高貴蔗與印度蔗、野生蔗及新幾內亞野生蔗親緣最為接近，跨屬比較時與芒屬序列最為接近。

關鍵字

紅甘蔗 ； 核糖體基因 ； 內轉錄間隔區

並列摘要

The internal transcribed spacer (ITS) was cloned with a primer pairs: 5'-GTC CCT GCC CTT TGT ACA-3' and 5'-CGC CGT TAC TAG GGG AAT CCT-3'. A total 821 bp were cloned including partial sequence of 18S ribosomal DNA flanking ITS region (5' end, 142 bp), full length ITS (587 bp), and partial sequence of 26S ribosomal DNA flanking ITS region (3' end, 92 bp). The ITS sequence of Saccharum officinarum is most closely related to S. spontaneum and S. robustum in the genus Sacharum, while most closely related to Erianthus spp. when it was compared across genus.

並列關鍵字

Saccharum officinarum ； ribosomal DNA ； ITS