本研究以清除DPPH自由基與FRAP法來測定四種香蜂草品種(TCA8901、TCA8902、TCA8903與TCA9101)之水萃凍乾物、袋茶浸出液以及精油等樣品之抗氧化能力。清除DPPH自由基能力之分析顯示,清除50% DPPH自由基之濃度(IC50)以香蜂草TCA8901 (43 μg/mL)之水萃凍乾物樣品表現最佳,其次依序為TCA9101(53 μg/mL)、TCA8902(55 μg/mL)及TCA8903(57 μg/mL),再者為袋茶浸出液(168 μg/mL);精油部分則皆表現不佳。水萃凍乾物樣品之FRAP分析方面,顯示香蜂草TCA9101之表現最佳,其1 g水萃凍物粉末之還原能力FRAP值相當於13.75 mg的維生素C,其他依序為TCA8901 (13.70 mg)、TCA8903 (12.54 mg)以及TCA8902(12.16 mg),再者為袋茶浸出液(1.83 mg);精油樣品之FRAP分析結果方面,香蜂草TCA9101之表現最佳,其1g精油之還原能力FRAP值相當於148.9 mg的BHT,其他依序為TCA8901(135.9 mg)、TCA8902(121.9 mg)以及TCA8903(103.7 mg)。整體而言,以品種TCA8901及TCA9101之抗氧化能力表現較佳。
The purpose of this study was to evaluate the antioxidant abilities of freeze-dried water extracts and essential oils from 4 species of Melissa officinalis L. (TCA8901, TCA8902, TCA8903 & TCA9101) and the tea bag of M. officinalis L., using scavenging DPPH and FRAP assays. In scavenging 50 % DPPH assay (IC50), freeze-dried water extract of TCA8901 had higher antioxidant activity (43 μg/mL), followed by TCA9101 (53 μg/mL), TCA8902 (55 μg/mL), TCA8903 (57 μg/mL), and tea bag liquor (168 μg/mL). However, no apparent antioxidant activity was observed with essential oils. In the FRAP assay, each gram of freeze-dried water extracts had different reducing capacity (FRAP value) in an order of TCA9101, TCA8901, TCA8903 and TCA8902, equivalent to 13.75, 13.70, 12.54 and 12.16 mg vitamin C, respectively. The FRAP value of tea bag liquor was equivalent to 1.83 mg vitamin C. Moreover, each gram of essential oils was found to have reducing capacity (FRAP value) in an order of TCA9101, TCA8901, TCA8902 and TCA8903, equivalent to 148.9, 135.9, 121.9 and 103.7 mg of BHT, respectively. Above all, M. officinalis species of TCA8901 and TCA9101 were found to have better antioxidant abilities in this study.