瓶苗出瓶適期對文心蘭幼苗生育之影響

Effect of Time to Deflask on the Growth and Development of Oncidium Hybrids Seedlings

陳姿翰(Tzu-Han Chen) ； 易美秀(Meei-Shiou Yih) ； 魏芳明(Fang-Ming Wei)

《臺中區農業改良場研究彙報》 112期 (2011/09) Pp. 15-24

https://doi.org/10.6956/BTDAIS.201109.0015

摘要

適合的瓶內培養週數為影響文心蘭出瓶後植株生長發育的重要因素。本研究針對4個文心蘭品種，分別比較不同培植週數出瓶時及出瓶栽培90天後之生長狀況，以期找出最佳的出瓶適期。Oncidium. Golden Shower 'Red Spots'及Onc. Gower Ramsey雖然於二次繼代16週出瓶時之鮮重最高，但栽培90天後之鮮重增加量較12週出瓶者低。Onc. Sharry Baby 'Sweet Fragrance'及Onc. Tdares Golden Mine 'Taichung No. 1 Gold Coins'則以二次繼代16週出瓶後之生長量最高。4品種皆以培養8週後出瓶之植株生長發育最差。培植週數過短，組培苗苗齡太小，其生長量低。隨著培養週數增加，培植體之鮮重也會隨之增加。株齡太大時，雖然出瓶鮮重高，但後續生長延緩。栽培環境對於出瓶後植株生長發育及存活率具有影響力，出瓶後栽培於日溫25℃、夜溫20℃，光強度300 μmol．m^(-2)．s^(-1)，光週期12 hr下之植株，生長情形均較對照組為佳。

關鍵字

文心蘭 ； 瓶內培養 ； 出瓶適期

並列摘要

Appropriate cultural duration is an important factor affecting the growth and development of deflasked plantlets. The objective of this study was to find optimal time to deflask according to the growth situation of seedlings at 90 days after deflask in 4 Oncidium cultivars. For the 4 cultivars tested, the plantlets had the worst growth efficiency was found when deflasked 8 weeks after subculture. Fresh weights of Onc. Golden shower 'Red spots' and Onc. Gower Ramsey plantlets were highest when deflasked 16 weeks after subculture. But 90 days after transplanting, the increase of fresh weight of plantlets was higher if they were deflasked 12 weeks after subculture. Growth increment of Onc. Sharry Baby 'Sweet Fragrance' and Onc. Tdares Golden Mine 'Taichung No.1 Gold Coins' plantlets was highest when deflasked 16 weeks after subculture. Fresh weights of plantlets were increased by the increasing of subculture weeks. Subculture with fewer subculture weeks, plantlets were too young to acclimate to the ex vitro environment, resulting in lower growth increment and lower assimilate accumilation. Growth of plantlets cultured in 25℃ day temperature, 20℃ night temperature, 300 μmol．m^(-2)．s^(-1), and 12 hours light cycle was better than that of control. Culture environment affected greatly the survival and growth of deflasked plantlets.

並列關鍵字

Oncidium ； in vitro culture ； optimal time to deflask