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菩提奇異球菌（Deinococcus ficus）CC-ZG207之羽毛分解液作為速效性液肥之研究
Study on the Characterization of Feather Degrading Solution by Deinococcus ficus CC-ZG207 as A Fast Release Liquid Fertilizer
摘要
羽毛為家禽類大宗之廢棄物。羽毛係由羽毛角蛋白質組成，由於角蛋白質之結構緊密及雙硫鍵含量豐富，因此不易分解。羽毛可作為土壤有機肥質料供作物養分吸收，然因羽毛分解速率緩慢，而無法立即提供作物生長初期養分吸收，若以微生物初步分解羽毛則可提高其養分釋放率。本研究以菩提奇異球菌（Deinococcus ficus）菌株CC-ZG207進行羽毛分解，發現以羽毛作為唯一碳氮源之培養基中，其角蛋白酶活性於培養的第4天達到最大為21.9 U/mL，羽毛分解率於培養的第2天至第8天，由64.8％增加至79.4％。菩提奇異球菌菌株CC-ZG207之羽毛分解液含有多種游離胺基酸，其中包含許多必需胺基酸，且胺基酸的含量以methionine含硫必需胺基酸為最多。本研究另分析羽毛分解後之培養液之EC、可溶性銨態氮、可溶性磷及可溶性鉀之含量分析，其結果顯示菩提奇異球菌菌株CC-ZG207分解羽毛後培養液之電導度值、可溶性銨態氮、可溶性磷及可溶性鉀較對照組皆明顯增加。本研究結果顯示菩提奇異球菌菌株CC-ZG207之羽毛分解液具有作為農業廢棄物開發應用的潛力，可應用於動物飼料添加或作為液態有機質肥料，且可加速羽毛廢棄物再利用之效率。
並列摘要
Feather is the waste product in poultry industry the main conponent of feather is keratin. However, keratin is reluctant to degrade because of its higher content in disulphide bond and compact keratin structure. Feather is rich in nitrogen and can be used as organic fertilizer resource, however the slow nutrient release makes it inadequate for using as plant nutrient at the initial growth stage. The pre-degradation by microorganism can increase the nutrient release in feather process. In this study, feather is applied as sole carbon and nitrogen source in the feather medium and to be degraded by Deinococcus ficus CC-ZG207, the maximum keratinase activity was 21.9 U/mL on day 4 and the feather degradation percentage was 64.8% on day 2 increased to 79.4% on day 8. Various free amino acids including essential ones were detected in the feather degrading solution by D. ficus CC-ZG207, and the most abundant among them was essential amino acid, methionine. The EC, solubilizing amino-nitrogen, phosphate and potassium were also increased accompany by feather degradation of D. ficus CC-ZG207. These results showed the feather-degrading solution of D. ficus CC-ZG207 has the potential to apply in the agriculture field such as fodder nutrient supplement and fast release fertilizer for accelerating the feather waste recycling.
延伸閱讀
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