MS培養基及N^6-Benzylaminopurine濃度對春石斛蘭莖節培養及植株生長之影響

MS Medium and Different Concentrations of N^6-Benzylaminopurine on Stem Segments Culture and Plant Growth of Nobile type Dendrobium

陳美齡(Mei-Ling Chen) ； 洪惠娟(Hui-Chuan Hung)

《臺中區農業改良場研究彙報》 118期 (2013/03) Pp. 1-10

摘要

本研究以3種春石斛蘭Den. Red Emperor（RE）、Den. Oriental Smile（C-12）及Den. Angel Baby‘Love Pocket’（PL）品種的莖節為試驗材料，探討不同培養基對春石斛蘭莖節萌芽及芽體生長之影響。由試驗結果顯示，經培養60日後，除了PL品種外，RE及C-12品種之春石斛蘭莖節培養的萌芽率因培養基中添加BAP而明顯增加，惟添加BAP 1或2 ppm處理間差異不大。將新芽體移至F2培養基培養30日後，不同品種春石斛蘭之植株生育特性顯現出各別差異，平均植株鮮重132～718 mg、株高10～32.6 mm、葉數2.2～5.9片、節數2～5.2節、芽數1～2.4個、根數0.8～5.1條及根長1.9～14.1 mm。綜合植株生長整體表現，以原培養在MS+1 ppm BAP的新芽體之植株生長發育較佳。

關鍵字

春石斛 ； 莖節培養

並列摘要

The objective of this study was to evaluate the effect of different media on stem segments culture of three nobile types Dendrobium, Red Emperor (RE), Oriental Smile (C-12) and Angel baby 'Love Pocket' (PL). Results showed that except cultivar PL, shoot regeneration rates of RE and C-12 cultivars were increased in media with BAP (N^6-Benzylaminopurine), but there was no significant difference between BAP 1 and 2 ppm treatments. The regenerated shoots were then transferred to F2 medium which had no plant growth regulators added. After 30 days of culture, different cultivars showed different growth characteristics; the plant fresh weight 132 ~ 718 mg, stem length 10 ~ 32.6 mm, number of leaves 2.2 ~ 5.9, number of nodes 2 ~ 5.2, number of buds 1 ~ 2.4, number of roots 0.8 ~ 5.1 and root length 1.9 ~ 14.1 mm. The explants which originally cultured in MS+1 ppm BAP media had better growth and development.

並列關鍵字

nobile type dendrobium ； stem segments culture ； N^6-Benzylaminopurine