臺灣李細菌性穿孔病菌之特性

Characterization of the Bacterial Spot Pathogen of Japanese Plum in Taiwan

黃冬青(Tung-Chin Huang) ； 沈原民(Yuan-Min Shen) ； 劉興隆(Hsing-Lung Liu) ； 趙佳鴻(Chia-Hung Chao)

《臺中區農業改良場研究彙報》 123期 (2014/06) Pp. 31-39

摘要

過去在臺灣針對薔薇科果樹細菌性穿孔病的記錄與報告甚少，本文以梨山李果實、葉片、枝條分離的三株李細菌性穿孔病菌（Xanthomonas arboricola pv. pruni）描述其形態、核酸序列特性、以及碳源利用之型態。觀察到細菌的形狀為桿狀，不產生孢子，大小0.8-1.0 x 1.6-2.7 μm，具有一條極生鞭毛。分離得到三株細菌的16S rDNA與已知的X. arboricola有99-100%的序列相同度。三株細菌的rpoD基因序列與已知X. arboricola pv. pruni序列皆100%相同，以rpoD基因序列特性可鑑定X. arboricola pv. pruni病原小種。分析此三株於臺灣分得之病原菌的碳源代謝活性，亦大致與X. arboricola相符，主要的差異為三株菌皆無法利用glycogen與acetic acid。本文首次以在臺灣取得的X. arboricola pv. pruni樣本，呈現細菌之形態、16S rDNA及rpoD序列、及碳源利用特性。

關鍵字

植物病原細菌 ； 果樹 ； 李 ； Biolog代謝指紋

並列摘要

Few studies in Taiwan focus on ＂Xanthomonas＂ bacterial spot disease of stone fruit. This article describes morphology, sequence characters, and carbon source utilization of ＂Xanthomonas arboricola pv. pruni＂ based on three bacterial isolates isolated from Japanese plum fruits, leaves, and branches in Lishan, Taiwan. The bacteria observed were rod shaped (0.8-1.0×1.6-2.7 μm), non-sporing, and with single polar flagellum. The 16S rDNA sequences of the three isolates shared 99-100% identities with those of known ＂X. arboricola＂. Sequences of ＂rpoD＂ gene of these isolates were 100% identical to those of known ＂X. arboricola pv. pruni＂. Thus, the ＂rpoD＂ gene sequences served as a trait for ＂X. arboricola pv. pruni＂ identification. Metabolic activities of the pathogens obtained in Taiwan generally fit in with metabolic data of ＂X. arboricola＂, except that all three isolates were unable to utilize glycogen and acetic acid. To our knowledge this is the first demonstration of bacterial morphology, sequences of 16S rDNA and ＂rpoD＂ gene, and carbon source utilization of ＂Xanthomonas arboricola pv. pruni＂ based on Taiwanese ＂X. arboricola pv. pruni＂ isolates.

並列關鍵字

Xanthomonas arboricola pv. pruni ； Plant Pathogenic Bacteria ； Japanese Plum ； Biolog Metabolic Fingerprint