放射菌應用於紅龍果廢棄枝條與羽毛轉化生成微生物肥料之研究

Application of Actinobacteria on Development of Biofertilizer with Utilization of Wasted Pitaya Cladode and Feather

曾宥綋(You-Hong Zeng) ； 賴文龍(Wen-Lung Lay) ； 郭雅紋(Ya-Wen Kuo) ； 陳鴻堂(Hong-Tang Chen)

《臺中區農業改良場研究彙報》 127期 (2015/06) Pp. 27-39

摘要

本研究篩選具分解羽毛及溶磷能力之節桿菌放線菌菌株K10（Arthrobacter sp. K10）及有效分解紅龍果枝條之鏈黴菌放線菌菌株CP3（Streptomyces sp. CP3），以應用於紅龍果廢棄枝條及羽毛再利用。研究顯示菌株K10可以羽毛做為生長所需之碳氮源，培養於礦物鹽羽毛培養基中（BHF）至第4天，羽毛分解率為63.7%，將其培養於10%（w/v）紅龍果枝條塊及1%（w/v）羽毛培養基（PIF），仍可有效分解羽毛，轉化生成銨離子及胺基酸，且分解液之電導度明顯增加，紅龍果枝條可做為菌株K10分解羽毛所需之養分，取代礦物鹽之功能。菌株K10具有溶解磷酸三鈣及磷礦石粉之功能，經培養於PIF培養基至第4天及第6天，其分解液菌數可達10^8 cfu/ml以上，且具有溶磷活性。紅龍果枝條分解菌CP3具有分解纖維及果膠能力，可有效分解紅龍果枝條，經由混合接種菌株K10，可增加分解紅龍果枝條及羽毛之效能，其分解液含有多種胺基酸，並以含硫胺基酸含量最多，具有相對易分解高氮養分特性，可使用於補充作物所需氮肥，並經由溶磷能力之菌株K10來提高土壤有效性磷含量，達成廢棄資材加值再利用。

關鍵字

放線菌 ； 紅龍果枝條 ； 羽毛分解 ； 溶磷菌

並列摘要

In order to recycle using wasted pitaya cladode and feather, the bacterium isolated as Streptomyces strain CP3 can efficiently degrade pitaya cladode and the bacterium isolated as Arthrobacter strain K10 had dual functions including feather degradation and phosphate solubilization. Feather used as the sole carbon and nitrogen source for strain K10, the feather degradation rate was 63.7% after incubating in medium contained mineral salts and feather (BHF medium) added to the 4th day. Feather can also be efficiently degraded by incubating strain K10 in 10% (w/v) pitaya cladode and 1% (w/v) feather medium (PIF medium), in which more ammonium ion and amino acids was producing to combine with EC value increase in the feather hydrolysates. The results showed that the nutrients in pitaya cladode substitute minerals during feather degradation. At 4th and 6th day of incubating strain K10 in PIF medium, the bacterial numbers in feather hydrolysates were more than 10^8 cfu/ml and were able to solublize calcium phosphate and rock phosphate. Cellulose and pectin-degrading Streptomyces strain CP3 can be used to increase degradation efficiency of pitaya cladode and feather by co-inoculating the strain K10 in the PIF medium, among the various amino acids produced, the concentration of sulfuric amino acids were higher. The inoculation of CP3 and K10 can be applied as relatively fast-released nitrogen fertilizer and enhancement of soil phosphorous bioavailability for value-added in the wasted pitaya cladode and feather reutilization.

並列關鍵字

Actinobacteria ； pitaya cladode ； feather degradation ； phosphate-solubilizing bacteria