葉用蘿蔔種原遺傳歧異度及耐熱性評估

Evaluation of Genetic Diversity and Heat Tolerance in Leafy Radish (＂Raphanus sativus＂ var. ＂oleiformis＂)

陳葦玲(We-Ling Chen) ； 楊雯如(Wen-Ju Yang)

《臺中區農業改良場研究彙報》 127期 (2015/06) Pp. 41-62

摘要

本研究為探討葉用蘿蔔種原之遺傳歧異度，並利用細胞膜熱穩定性（cell membrane thermostability, CMT）評估種原耐熱性。21個葉用蘿蔔品種（系）在營養性狀及春化需求皆具顯著差異性，以21條區間簡單序列重複（inter simple sequence repeats, ISSR）引子可擴增出292個條帶，平均每個引子可產生13.9個條帶，其中76.7%為穩定具再現性之多型性條帶，顯示葉用蘿蔔族群變異性極高，群集分析結果和植株營養、開花性狀及種原來源具相關性。另子葉以50℃水浴處理30分鐘之熱相對傷害值（relative injury, RI）能區別種原間之耐熱性差異，且與高溫下產量減少比例具高度相關性，RI值低於50%可作為耐熱性篩選指標，進行苗期早期選拔，以加速耐熱葉用蘿蔔選育。

關鍵字

葉用蘿蔔 ； 種原 ； 區間簡單序列重複 ； 相對傷害值 ； 耐熱性

並列摘要

The study aimed to investigate the genetic diversity of leafy radish (＂Raphanus sativus＂ L. var. ＂oleiformis＂ Pers.) germplasm and evaluate their heat tolerance by cell membrane thermostability (CMT). The significant variance of vegetative and vernalizative characteristics was found among 21 collected leafy radish cultivars (lines). Based on the inter simple sequence repeats (ISSR) analysis, 292 bands were generated from 21 primers with an average of 13.9 bands per primer. There were 76.7% polymerase chain reaction (PCR) products being reproducible, polymorphic and revealed high genetic diversity in leafy radish accessions. The results of cluster analysis showed that the relationship between plant vegetative, reproductive traits and their origins were close. Besides, relative injury (RI) of cotyledon tissues after 50°C water bath, for 30 mins had high correlation with yield reduction caused by high temperature. This result was suggested RI value lower than 50% as a heat tolerant selecting index for leafy radish genotypes during seedling stage. It can be useful to facilitate heat tolerant breeding efficiency of leafy radish in the future.

並列關鍵字

＂Raphanus sativus＂ L. var. ＂oleiformis＂ Pers. ； germplasm ； inter simple sequence repeat ； relative injury value ； heat tolerance