The aim of this study was to investigate the impact of iron on the growth of 'Tainung No.1' passion fruit (Passiflora edulis x P. edulis f. flavicarpa), focusing on the effect of iron deficiency. Results showed that iron deficiency directly inhibited the growth of passion fruit. The plant height, number of lateral buds, stem weight, leaf iron and manganese contents were significantly higher in the treatment with 0.04 mM (EDTA-Fe, added 3 times a week) than those in the treatment with 0 mM. Meanwhile, the soluble solid content in the 0 mM treatment was significantly lower than those in the control group (0.02 mM) and 0.04 mM treatment. Iron fertilizer has a huge impact on the vegetative growth and fruit quality of passion fruit. In order to improve the growth of 'Tainung No.1'passion fruit and produce high-quality fruits we should pay attention to iron supplementation.